Yellow fever virus capsid protein is a potent suppressor of RNA silencing that binds double-stranded RNA

Glady Hazitha Samuel, Michael R Wiley, Atif Badawi, Zach N. Adelman, Kevin M. Myles

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Mosquito-borne flaviviruses, including yellow fever virus (YFV), Zika virus (ZIKV), and West Nile virus (WNV), profoundly affect human health. The successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. Similar to other invertebrate animals and plants, the mosquito's RNA silencing pathway comprises its primary antiviral defense. Although a diverse range of plant and insect viruses has been found to encode suppressors of RNA silencing, the mechanisms by which flaviviruses antagonize antiviral small RNA pathways in disease vectors are unknown. Here we describe a viral suppressor of RNA silencing (VSR) encoded by the prototype flavivirus, YFV. We show that the YFV capsid (YFC) protein inhibits RNA silencing in the mosquito Aedes aegypti by interfering with Dicer. This VSR activity appears to be broadly conserved in the C proteins of other medically important flaviviruses, including that of ZIKV. These results suggest that a molecular "arms race" between vector and pathogen underlies the continued existence of flaviviruses in nature.

Original languageEnglish (US)
Pages (from-to)13863-13868
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number48
DOIs
StatePublished - Nov 29 2016
Externally publishedYes

Fingerprint

Yellow fever virus
Flavivirus
Double-Stranded RNA
Capsid Proteins
RNA Interference
Culicidae
Viral RNA
Antiviral Agents
Feline Panleukopenia
Insect Viruses
Plant RNA
Disease Vectors
Plant Viruses
West Nile virus
Aedes
Invertebrates
Protein C
RNA
Viruses
Health

Keywords

  • Capsid
  • Flavivirus
  • RNA interference
  • Yellow fever virus
  • Zika virus

ASJC Scopus subject areas

  • General

Cite this

Yellow fever virus capsid protein is a potent suppressor of RNA silencing that binds double-stranded RNA. / Samuel, Glady Hazitha; Wiley, Michael R; Badawi, Atif; Adelman, Zach N.; Myles, Kevin M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 48, 29.11.2016, p. 13863-13868.

Research output: Contribution to journalArticle

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