Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends.

Kelly M. Trujillo, Dong Hyun Roh, Ling Chen, Stephen Van Komen, Alan Tomkinson, Patrick Sung

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Saccharomyces cerevisiae Rad50, Mre11, and Xrs2 proteins are involved in homologous recombination, non-homologous end-joining, DNA damage checkpoint signaling, and telomere maintenance. These proteins form a stable complex that has nuclease, DNA binding, and DNA end recognition activities. Of the components of the Rad50.Mre11.Xrs2 complex, Xrs2 is the least characterized. The available evidence is consistent with the idea that Xrs2 recruits other protein factors in reactions that pertain to the biological functions of the Rad50.Mre11.Xrs2 complex. Here we present biochemical evidence that Xrs2 has an associated DNA-binding activity that is specific for DNA structures. We also define the contributions of Xrs2 to the activities of the Rad50.Mre11.Xrs2 complex. Importantly, we demonstrate that Xrs2 is critical for targeting of Rad50 and Mre11 to DNA ends. Thus, Xrs2 likely plays a direct role in the engagement of DNA substrates by the Rad50. Mre11.Xrs2 complex in various biological processes.

Original languageEnglish (US)
Pages (from-to)48957-48964
Number of pages8
JournalThe Journal of biological chemistry
Volume278
Issue number49
DOIs
StatePublished - Dec 5 2003

Fingerprint

Yeast
Yeasts
DNA
Biological Phenomena
Proteins
Deoxyribonucleases
Homologous Recombination
Telomere
DNA Damage
Saccharomyces cerevisiae
Joining
Maintenance
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Trujillo, K. M., Roh, D. H., Chen, L., Van Komen, S., Tomkinson, A., & Sung, P. (2003). Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. The Journal of biological chemistry, 278(49), 48957-48964. https://doi.org/10.1074/jbc.M309877200

Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. / Trujillo, Kelly M.; Roh, Dong Hyun; Chen, Ling; Van Komen, Stephen; Tomkinson, Alan; Sung, Patrick.

In: The Journal of biological chemistry, Vol. 278, No. 49, 05.12.2003, p. 48957-48964.

Research output: Contribution to journalArticle

Trujillo, KM, Roh, DH, Chen, L, Van Komen, S, Tomkinson, A & Sung, P 2003, 'Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends.', The Journal of biological chemistry, vol. 278, no. 49, pp. 48957-48964. https://doi.org/10.1074/jbc.M309877200
Trujillo KM, Roh DH, Chen L, Van Komen S, Tomkinson A, Sung P. Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. The Journal of biological chemistry. 2003 Dec 5;278(49):48957-48964. https://doi.org/10.1074/jbc.M309877200
Trujillo, Kelly M. ; Roh, Dong Hyun ; Chen, Ling ; Van Komen, Stephen ; Tomkinson, Alan ; Sung, Patrick. / Yeast xrs2 binds DNA and helps target rad50 and mre11 to DNA ends. In: The Journal of biological chemistry. 2003 ; Vol. 278, No. 49. pp. 48957-48964.
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