Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2

Jinglin L. Xie, Iryna Bohovych, Erin O.Y. Wong, Jean Philippe Lambert, Anne Claude Gingras, Oleh Khalimonchuk, Leah E. Cowen, Michelle D. Leach

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mitochondria underpin metabolism, bioenergetics, signalling, development and cell death in eukaryotes. Most of the ~1,000 yeast mitochondrial proteins are encoded in the nucleus and synthesised as precursors in the cytosol, with mitochondrial import facilitated by molecular chaperones. Here, we focus on the Hsp40 chaperone Ydj1 in the fungal pathogen Candida albicans, finding that it is localised to both the cytosol and outer mitochondrial membrane, and is required for cellular stress responses and for filamentation, a key virulence trait. Mapping the Ydj1 protein interaction network highlighted connections with co-chaperones and regulators of filamentation. Furthermore, the mitochondrial processing peptidases Mas1 and Mas2 were highly enriched for interaction with Ydj1. Additional analysis demonstrated that loss of MAS1, MAS2 or YDJ1 perturbs mitochondrial morphology and function. Deletion of YDJ1 impairs import of Su9, a protein that is cleaved to a mature form by Mas1 and Mas2. Thus, we highlight a novel role for Ydj1 in cellular morphogenesis, stress responses, and mitochondrial import in the fungal kingdom.

Original languageEnglish (US)
Pages (from-to)342-361
Number of pages20
JournalMicrobial Cell
Volume4
Issue number10
DOIs
StatePublished - Oct 2017

Fingerprint

Mitochondrial Proteins
Morphogenesis
Cytosol
Protein Interaction Maps
Mitochondria
Molecular Chaperones
Fungal Proteins
Candida
Mitochondrial Membranes
Pathogens
Cell death
Eukaryota
Candida albicans
Metabolism
Energy Metabolism
Virulence
Proteins
Cell Death
Membranes
mitochondrial processing peptidase

Keywords

  • Candida albicans
  • Heat shock
  • Mitochondria
  • Mitochondrial processing peptidases
  • Morphogenesis
  • Stress

ASJC Scopus subject areas

  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2. / Xie, Jinglin L.; Bohovych, Iryna; Wong, Erin O.Y.; Lambert, Jean Philippe; Gingras, Anne Claude; Khalimonchuk, Oleh; Cowen, Leah E.; Leach, Michelle D.

In: Microbial Cell, Vol. 4, No. 10, 10.2017, p. 342-361.

Research output: Contribution to journalArticle

Xie, Jinglin L. ; Bohovych, Iryna ; Wong, Erin O.Y. ; Lambert, Jean Philippe ; Gingras, Anne Claude ; Khalimonchuk, Oleh ; Cowen, Leah E. ; Leach, Michelle D. / Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2. In: Microbial Cell. 2017 ; Vol. 4, No. 10. pp. 342-361.
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