Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast

Jaekwon Lee, Christian Godon, Gilles Lagniel, Daniel Spector, Jérome Garin, Jean Labarre, Michel B. Toledano

Research output: Contribution to journalArticle

419 Citations (Scopus)

Abstract

Yap1 and Skn7 are two yeast transcriptional regulators that co-operate to activate thioredoxin (TRX2) and thioredoxin reductase (TRR1) in response to redox stress signals. Although they are both important for resistance to H2O2, only Yap1 is important for cadmium resistance, whereas Skn7 has a negative effect upon this response. The respective roles of Yap1 and Skn7 in the induction of defense genes by H2O2 were analyzed by two-dimensional gel electrophoresis. Yap1 controls a large oxidative stress response regulon of at least 32 proteins. Fifteen of these proteins also require the presence of Skn7 for their induction by H2O2. Although about half of the Yap1 target genes do not contain a consensus Yap1 recognition motif, the control of one such gene, TSA1, involves the binding of Yap1 and Skn7 to its promoter in vitro. The co-operative control of the oxidative stress response by Yap1 and Skn7 delineates two gene subsets. Remarkably, these two gene subsets separate antioxidant scavenging enzymes from the metabolic pathways regenerating the main cellular reducing power, glutathione and NADPH. Such a specialization may explain, at least in part, the dissociated function of Yap1 and Skn7 in H2O2 and cadmium resistance.

Original languageEnglish (US)
Pages (from-to)16040-16046
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number23
DOIs
StatePublished - Jun 4 1999

Fingerprint

Regulon
Oxidative stress
Yeast
Oxidative Stress
Genes
Yeasts
Cadmium
Thioredoxin-Disulfide Reductase
Thioredoxins
Scavenging
Electrophoresis, Gel, Two-Dimensional
Metabolic Networks and Pathways
Electrophoresis
NADP
Oxidation-Reduction
Glutathione
Proteins
Antioxidants
Gels
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lee, J., Godon, C., Lagniel, G., Spector, D., Garin, J., Labarre, J., & Toledano, M. B. (1999). Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. Journal of Biological Chemistry, 274(23), 16040-16046. https://doi.org/10.1074/jbc.274.23.16040

Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. / Lee, Jaekwon; Godon, Christian; Lagniel, Gilles; Spector, Daniel; Garin, Jérome; Labarre, Jean; Toledano, Michel B.

In: Journal of Biological Chemistry, Vol. 274, No. 23, 04.06.1999, p. 16040-16046.

Research output: Contribution to journalArticle

Lee, J, Godon, C, Lagniel, G, Spector, D, Garin, J, Labarre, J & Toledano, MB 1999, 'Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast', Journal of Biological Chemistry, vol. 274, no. 23, pp. 16040-16046. https://doi.org/10.1074/jbc.274.23.16040
Lee, Jaekwon ; Godon, Christian ; Lagniel, Gilles ; Spector, Daniel ; Garin, Jérome ; Labarre, Jean ; Toledano, Michel B. / Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 23. pp. 16040-16046.
@article{94bcaba9bbfa49eb9cc269cd3e876977,
title = "Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast",
abstract = "Yap1 and Skn7 are two yeast transcriptional regulators that co-operate to activate thioredoxin (TRX2) and thioredoxin reductase (TRR1) in response to redox stress signals. Although they are both important for resistance to H2O2, only Yap1 is important for cadmium resistance, whereas Skn7 has a negative effect upon this response. The respective roles of Yap1 and Skn7 in the induction of defense genes by H2O2 were analyzed by two-dimensional gel electrophoresis. Yap1 controls a large oxidative stress response regulon of at least 32 proteins. Fifteen of these proteins also require the presence of Skn7 for their induction by H2O2. Although about half of the Yap1 target genes do not contain a consensus Yap1 recognition motif, the control of one such gene, TSA1, involves the binding of Yap1 and Skn7 to its promoter in vitro. The co-operative control of the oxidative stress response by Yap1 and Skn7 delineates two gene subsets. Remarkably, these two gene subsets separate antioxidant scavenging enzymes from the metabolic pathways regenerating the main cellular reducing power, glutathione and NADPH. Such a specialization may explain, at least in part, the dissociated function of Yap1 and Skn7 in H2O2 and cadmium resistance.",
author = "Jaekwon Lee and Christian Godon and Gilles Lagniel and Daniel Spector and J{\'e}rome Garin and Jean Labarre and Toledano, {Michel B.}",
year = "1999",
month = "6",
day = "4",
doi = "10.1074/jbc.274.23.16040",
language = "English (US)",
volume = "274",
pages = "16040--16046",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "23",

}

TY - JOUR

T1 - Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast

AU - Lee, Jaekwon

AU - Godon, Christian

AU - Lagniel, Gilles

AU - Spector, Daniel

AU - Garin, Jérome

AU - Labarre, Jean

AU - Toledano, Michel B.

PY - 1999/6/4

Y1 - 1999/6/4

N2 - Yap1 and Skn7 are two yeast transcriptional regulators that co-operate to activate thioredoxin (TRX2) and thioredoxin reductase (TRR1) in response to redox stress signals. Although they are both important for resistance to H2O2, only Yap1 is important for cadmium resistance, whereas Skn7 has a negative effect upon this response. The respective roles of Yap1 and Skn7 in the induction of defense genes by H2O2 were analyzed by two-dimensional gel electrophoresis. Yap1 controls a large oxidative stress response regulon of at least 32 proteins. Fifteen of these proteins also require the presence of Skn7 for their induction by H2O2. Although about half of the Yap1 target genes do not contain a consensus Yap1 recognition motif, the control of one such gene, TSA1, involves the binding of Yap1 and Skn7 to its promoter in vitro. The co-operative control of the oxidative stress response by Yap1 and Skn7 delineates two gene subsets. Remarkably, these two gene subsets separate antioxidant scavenging enzymes from the metabolic pathways regenerating the main cellular reducing power, glutathione and NADPH. Such a specialization may explain, at least in part, the dissociated function of Yap1 and Skn7 in H2O2 and cadmium resistance.

AB - Yap1 and Skn7 are two yeast transcriptional regulators that co-operate to activate thioredoxin (TRX2) and thioredoxin reductase (TRR1) in response to redox stress signals. Although they are both important for resistance to H2O2, only Yap1 is important for cadmium resistance, whereas Skn7 has a negative effect upon this response. The respective roles of Yap1 and Skn7 in the induction of defense genes by H2O2 were analyzed by two-dimensional gel electrophoresis. Yap1 controls a large oxidative stress response regulon of at least 32 proteins. Fifteen of these proteins also require the presence of Skn7 for their induction by H2O2. Although about half of the Yap1 target genes do not contain a consensus Yap1 recognition motif, the control of one such gene, TSA1, involves the binding of Yap1 and Skn7 to its promoter in vitro. The co-operative control of the oxidative stress response by Yap1 and Skn7 delineates two gene subsets. Remarkably, these two gene subsets separate antioxidant scavenging enzymes from the metabolic pathways regenerating the main cellular reducing power, glutathione and NADPH. Such a specialization may explain, at least in part, the dissociated function of Yap1 and Skn7 in H2O2 and cadmium resistance.

UR - http://www.scopus.com/inward/record.url?scp=0033523113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033523113&partnerID=8YFLogxK

U2 - 10.1074/jbc.274.23.16040

DO - 10.1074/jbc.274.23.16040

M3 - Article

C2 - 10347154

AN - SCOPUS:0033523113

VL - 274

SP - 16040

EP - 16046

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 23

ER -