Xylo-oligosaccharides inhibit pathogen adhesion to enterocytes invitro

Tine Ebersbach, Jens Bo Andersen, Anders Bergström, Robert W. Hutkins, Tine Rask Licht

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We previously reported that the non-digestible carbohydrates inulin and apple pectin promoted Listeria monocytogenes infection in guinea pigs, whereas xylo- and galacto-oligosaccharides (XOS and GOS), prevented infection by this pathogen. In the present study, mechanisms that could explain the previous invivo observations were explored. Mixing bacterial cultures with XOS significantly (P<0.05) decreased the ability of two out of three strains of L. monocytogenes to adhere to Caco-2 cells. Additionally, 2h incubation with XOS followed by washing of the bacteria significantly (P<0.05) decreased the ability of all three strains to adhere to Caco-2 cells. Consistently, expression of the adhesion-relevant genes inlA and lap was reduced by the presence of XOS. The observation that XOS inhibit the adhesion of Listeria to the intestinal epithelium invitro may explain the reported preventive effect of XOS on Listeria infection in guinea pigs invivo, while the preventive effect of GOS was not explicable by the assays chosen here.

Original languageEnglish (US)
Pages (from-to)22-27
Number of pages6
JournalResearch in Microbiology
Volume163
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Listeriosis
Caco-2 Cells
Enterocytes
Listeria monocytogenes
Oligosaccharides
Guinea Pigs
Listeria
Inulin
Malus
Intestinal Mucosa
Carbohydrates
Bacteria
Infection
Genes
pectin

Keywords

  • Adhesion
  • Listeria monocytogenes
  • Prebiotics
  • QPCR
  • Virulence expression

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Xylo-oligosaccharides inhibit pathogen adhesion to enterocytes invitro. / Ebersbach, Tine; Andersen, Jens Bo; Bergström, Anders; Hutkins, Robert W.; Licht, Tine Rask.

In: Research in Microbiology, Vol. 163, No. 1, 01.01.2012, p. 22-27.

Research output: Contribution to journalArticle

Ebersbach, Tine ; Andersen, Jens Bo ; Bergström, Anders ; Hutkins, Robert W. ; Licht, Tine Rask. / Xylo-oligosaccharides inhibit pathogen adhesion to enterocytes invitro. In: Research in Microbiology. 2012 ; Vol. 163, No. 1. pp. 22-27.
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