5 Citations (Scopus)

Abstract

Background: The cellular infiltrate in xenografts suggests that macrophages may be involved in xenograft rejection. However, the precise role of macrophages in xenograft rejection has not yet been fully addressed. Methods: Xenogeneic rat skin grafts were transplanted to macrophage colony, stimulating factor (M-CSF)/macrophage-deficient osteopetrotic ([OP]-/-) and wild-type control mice. Skin graft survival and antidonor rat humoral responses were quantified. Results: Xenogeneic rat skin grafts survived 13 days in wild-type control mice, survival of rat skin grafts was significantly prolonged to 24 days in [OP]-/- mice (P < 0.01). Similar results were observed in sensitized [OP]-/- and control mouse recipients, showing markedly prolonged rat skin graft survival in [OP]-/- mice. Levels of T-cell-dependent antirat antibodies [immunoglobulin G (IgG)2a and IgG3] in sera of [OP]-/- mice were significantly lower than that of control mice 2 weeks post-rat skin grafting. The proliferative responses to xenogeneic rats not to allogeneic mouse stimulation of T cells from [OP]-/- mice were significantly lower than that of wild-type mice. However, neutrilization of M-CSF by anti-M-CSF monoclonal antibody (mAb) or the addition of M-CSF to the in vitro culture systems of wild-type or [OP]-/- mouse T-responder cells, respectively, did not significantly change proliferative responses and cytolytic function against xenogeneic rat targets of wild-type or [OP]-/- mouse T-responder cells. Conclusions: The in vitro data indicate that M-CSF does not directly regulate cellular immune responses to xenoantigens. The present studies indicate that macrophages may play an important role in immune rejection of xenografts. The precise role of macrophages in xenograft rejection should be further investigated.

Original languageEnglish (US)
Pages (from-to)232-239
Number of pages8
JournalXenotransplantation
Volume10
Issue number3
DOIs
StatePublished - May 1 2003

Fingerprint

Macrophage Colony-Stimulating Factor
Graft Rejection
Macrophages
Skin
Heterografts
Graft Survival
Transplants
Immunoglobulin G
Heterophile Antigens
T-Lymphocytes
Skin Transplantation
Cellular Immunity
Monoclonal Antibodies

Keywords

  • Graft rejection
  • Immune response
  • Macrophage
  • Xenotransplantation

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Xenogeneic skin graft rejection in M-CSF/macrophage deficient osteopetrotic mice. / Zhao, Yong; Xiong, Wanfen; Yang, Tianyu; Prall, Amy; Baxter, Bernard Timothy; Langnas, Alan Norman.

In: Xenotransplantation, Vol. 10, No. 3, 01.05.2003, p. 232-239.

Research output: Contribution to journalArticle

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title = "Xenogeneic skin graft rejection in M-CSF/macrophage deficient osteopetrotic mice",
abstract = "Background: The cellular infiltrate in xenografts suggests that macrophages may be involved in xenograft rejection. However, the precise role of macrophages in xenograft rejection has not yet been fully addressed. Methods: Xenogeneic rat skin grafts were transplanted to macrophage colony, stimulating factor (M-CSF)/macrophage-deficient osteopetrotic ([OP]-/-) and wild-type control mice. Skin graft survival and antidonor rat humoral responses were quantified. Results: Xenogeneic rat skin grafts survived 13 days in wild-type control mice, survival of rat skin grafts was significantly prolonged to 24 days in [OP]-/- mice (P < 0.01). Similar results were observed in sensitized [OP]-/- and control mouse recipients, showing markedly prolonged rat skin graft survival in [OP]-/- mice. Levels of T-cell-dependent antirat antibodies [immunoglobulin G (IgG)2a and IgG3] in sera of [OP]-/- mice were significantly lower than that of control mice 2 weeks post-rat skin grafting. The proliferative responses to xenogeneic rats not to allogeneic mouse stimulation of T cells from [OP]-/- mice were significantly lower than that of wild-type mice. However, neutrilization of M-CSF by anti-M-CSF monoclonal antibody (mAb) or the addition of M-CSF to the in vitro culture systems of wild-type or [OP]-/- mouse T-responder cells, respectively, did not significantly change proliferative responses and cytolytic function against xenogeneic rat targets of wild-type or [OP]-/- mouse T-responder cells. Conclusions: The in vitro data indicate that M-CSF does not directly regulate cellular immune responses to xenoantigens. The present studies indicate that macrophages may play an important role in immune rejection of xenografts. The precise role of macrophages in xenograft rejection should be further investigated.",
keywords = "Graft rejection, Immune response, Macrophage, Xenotransplantation",
author = "Yong Zhao and Wanfen Xiong and Tianyu Yang and Amy Prall and Baxter, {Bernard Timothy} and Langnas, {Alan Norman}",
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T1 - Xenogeneic skin graft rejection in M-CSF/macrophage deficient osteopetrotic mice

AU - Zhao, Yong

AU - Xiong, Wanfen

AU - Yang, Tianyu

AU - Prall, Amy

AU - Baxter, Bernard Timothy

AU - Langnas, Alan Norman

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Background: The cellular infiltrate in xenografts suggests that macrophages may be involved in xenograft rejection. However, the precise role of macrophages in xenograft rejection has not yet been fully addressed. Methods: Xenogeneic rat skin grafts were transplanted to macrophage colony, stimulating factor (M-CSF)/macrophage-deficient osteopetrotic ([OP]-/-) and wild-type control mice. Skin graft survival and antidonor rat humoral responses were quantified. Results: Xenogeneic rat skin grafts survived 13 days in wild-type control mice, survival of rat skin grafts was significantly prolonged to 24 days in [OP]-/- mice (P < 0.01). Similar results were observed in sensitized [OP]-/- and control mouse recipients, showing markedly prolonged rat skin graft survival in [OP]-/- mice. Levels of T-cell-dependent antirat antibodies [immunoglobulin G (IgG)2a and IgG3] in sera of [OP]-/- mice were significantly lower than that of control mice 2 weeks post-rat skin grafting. The proliferative responses to xenogeneic rats not to allogeneic mouse stimulation of T cells from [OP]-/- mice were significantly lower than that of wild-type mice. However, neutrilization of M-CSF by anti-M-CSF monoclonal antibody (mAb) or the addition of M-CSF to the in vitro culture systems of wild-type or [OP]-/- mouse T-responder cells, respectively, did not significantly change proliferative responses and cytolytic function against xenogeneic rat targets of wild-type or [OP]-/- mouse T-responder cells. Conclusions: The in vitro data indicate that M-CSF does not directly regulate cellular immune responses to xenoantigens. The present studies indicate that macrophages may play an important role in immune rejection of xenografts. The precise role of macrophages in xenograft rejection should be further investigated.

AB - Background: The cellular infiltrate in xenografts suggests that macrophages may be involved in xenograft rejection. However, the precise role of macrophages in xenograft rejection has not yet been fully addressed. Methods: Xenogeneic rat skin grafts were transplanted to macrophage colony, stimulating factor (M-CSF)/macrophage-deficient osteopetrotic ([OP]-/-) and wild-type control mice. Skin graft survival and antidonor rat humoral responses were quantified. Results: Xenogeneic rat skin grafts survived 13 days in wild-type control mice, survival of rat skin grafts was significantly prolonged to 24 days in [OP]-/- mice (P < 0.01). Similar results were observed in sensitized [OP]-/- and control mouse recipients, showing markedly prolonged rat skin graft survival in [OP]-/- mice. Levels of T-cell-dependent antirat antibodies [immunoglobulin G (IgG)2a and IgG3] in sera of [OP]-/- mice were significantly lower than that of control mice 2 weeks post-rat skin grafting. The proliferative responses to xenogeneic rats not to allogeneic mouse stimulation of T cells from [OP]-/- mice were significantly lower than that of wild-type mice. However, neutrilization of M-CSF by anti-M-CSF monoclonal antibody (mAb) or the addition of M-CSF to the in vitro culture systems of wild-type or [OP]-/- mouse T-responder cells, respectively, did not significantly change proliferative responses and cytolytic function against xenogeneic rat targets of wild-type or [OP]-/- mouse T-responder cells. Conclusions: The in vitro data indicate that M-CSF does not directly regulate cellular immune responses to xenoantigens. The present studies indicate that macrophages may play an important role in immune rejection of xenografts. The precise role of macrophages in xenograft rejection should be further investigated.

KW - Graft rejection

KW - Immune response

KW - Macrophage

KW - Xenotransplantation

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