X-ray absorption spectroscopy of cuprous-thiolate clusters in Saccharomyces cerevisiae metallothionein

Limei Zhang, Ingrid J. Pickering, Dennis R. Winge, Graham N. George

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Copper (Cu) metallothioneins are cuprous-thiolate proteins that contain multimetallic clusters, and are thought to have dual functions of Cu storage and Cu detoxification. We have used a combination of X-ray absorption spectroscopy (XAS) and density-functional theory (DFT) to investigate the nature of Cu binding to Saccharomyces cerevisiae metallothionein. We found that the XAS of metallothionein prepared, containing a full complement of Cu, was quantitatively consistent with the crystal structure, and that reconstitution of the apo-metallothionein with stoichiometric Cu results in the formation of a tetracopper cluster, indicating cooperative binding of the Cu ions by the metallothionein.

Original languageEnglish (US)
Pages (from-to)2042-2049
Number of pages8
JournalChemistry and Biodiversity
Volume5
Issue number10
DOIs
StatePublished - Dec 1 2008

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X-Ray Absorption Spectroscopy
X ray absorption spectroscopy
Metallothionein
Yeast
Saccharomyces cerevisiae
Detoxification
Density functional theory
Copper
Crystal structure
Ions
Proteins

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Molecular Medicine
  • Molecular Biology

Cite this

X-ray absorption spectroscopy of cuprous-thiolate clusters in Saccharomyces cerevisiae metallothionein. / Zhang, Limei; Pickering, Ingrid J.; Winge, Dennis R.; George, Graham N.

In: Chemistry and Biodiversity, Vol. 5, No. 10, 01.12.2008, p. 2042-2049.

Research output: Contribution to journalArticle

Zhang, Limei ; Pickering, Ingrid J. ; Winge, Dennis R. ; George, Graham N. / X-ray absorption spectroscopy of cuprous-thiolate clusters in Saccharomyces cerevisiae metallothionein. In: Chemistry and Biodiversity. 2008 ; Vol. 5, No. 10. pp. 2042-2049.
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