Wound healing properties of Indian propolis studied on excision wound-induced rats

S. Iyyam Pillai, P. Palsamy, S. Subramanian, M. Kandaswamy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Context: In traditional medicine propolis is widely used for the treatment of various ailments including ulcer and wound healing. The phytochemical screening of Indian propolis indicates the presence of biologically active ingredients in appreciable amounts. In the absence of systematic evaluation of wound healing properties of Indian propolis in the literature, the present study was undertaken. Objective: The aim of this study was to evaluate the wound healing potential of Indian propolis on excision wounds induced in experimental rats. Materials and methods: Excision wounds were created in male Wistar rats and were treated with Indian propolis ointment (nitrofurazone was used as a reference drug - widely used for wound healing) for a period of 14 days. Control rats were treated with petroleum jelly. The parameters analyzed include wound contraction, hydroxyproline, hexosamine, uronic acid, total protein, DNA, and RNA. Results: Topical application of propolis ointment for 14 days significantly improved the wound contraction when compared to the control group of rats. The determination of hydroxyproline, hexosamine, uronic acid, DNA, RNA and protein levels in the wound matrix revealed the pro-healing effects of propolis. The results obtained were comparable with nitrofurazone. Discussion and conclusion: It appears that the ethanol extract of Indian propolis possesses significant pro-healing activity by accelerating the healing process at various phases of tissue repair. The presence of biologically active ingredients such as flavonoids, phenolic acids, terpenes, benzoic acids, amino acids and vitamins, etc. in Indian propolis may readily account for the observed prophylactic action of propolis in wound healing.

Original languageEnglish (US)
Pages (from-to)1198-1206
Number of pages9
JournalPharmaceutical Biology
Volume48
Issue number11
DOIs
StatePublished - Nov 1 2010

Fingerprint

Propolis
Wound Healing
Wounds and Injuries
Nitrofurazone
Uronic Acids
Hexosamines
Hydroxyproline
Ointments
RNA
Petrolatum
Benzoates
DNA
Terpenes
Phytochemicals
Traditional Medicine
Flavonoids
Vitamins
Ulcer
Wistar Rats
Proteins

Keywords

  • Excision wound
  • Flavonoids
  • Hydroxyproline
  • Indian propolis
  • Wound healing

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Wound healing properties of Indian propolis studied on excision wound-induced rats. / Iyyam Pillai, S.; Palsamy, P.; Subramanian, S.; Kandaswamy, M.

In: Pharmaceutical Biology, Vol. 48, No. 11, 01.11.2010, p. 1198-1206.

Research output: Contribution to journalArticle

Iyyam Pillai, S. ; Palsamy, P. ; Subramanian, S. ; Kandaswamy, M. / Wound healing properties of Indian propolis studied on excision wound-induced rats. In: Pharmaceutical Biology. 2010 ; Vol. 48, No. 11. pp. 1198-1206.
@article{102a36cf851c4b44b03655c1f8b4e1d2,
title = "Wound healing properties of Indian propolis studied on excision wound-induced rats",
abstract = "Context: In traditional medicine propolis is widely used for the treatment of various ailments including ulcer and wound healing. The phytochemical screening of Indian propolis indicates the presence of biologically active ingredients in appreciable amounts. In the absence of systematic evaluation of wound healing properties of Indian propolis in the literature, the present study was undertaken. Objective: The aim of this study was to evaluate the wound healing potential of Indian propolis on excision wounds induced in experimental rats. Materials and methods: Excision wounds were created in male Wistar rats and were treated with Indian propolis ointment (nitrofurazone was used as a reference drug - widely used for wound healing) for a period of 14 days. Control rats were treated with petroleum jelly. The parameters analyzed include wound contraction, hydroxyproline, hexosamine, uronic acid, total protein, DNA, and RNA. Results: Topical application of propolis ointment for 14 days significantly improved the wound contraction when compared to the control group of rats. The determination of hydroxyproline, hexosamine, uronic acid, DNA, RNA and protein levels in the wound matrix revealed the pro-healing effects of propolis. The results obtained were comparable with nitrofurazone. Discussion and conclusion: It appears that the ethanol extract of Indian propolis possesses significant pro-healing activity by accelerating the healing process at various phases of tissue repair. The presence of biologically active ingredients such as flavonoids, phenolic acids, terpenes, benzoic acids, amino acids and vitamins, etc. in Indian propolis may readily account for the observed prophylactic action of propolis in wound healing.",
keywords = "Excision wound, Flavonoids, Hydroxyproline, Indian propolis, Wound healing",
author = "{Iyyam Pillai}, S. and P. Palsamy and S. Subramanian and M. Kandaswamy",
year = "2010",
month = "11",
day = "1",
doi = "10.3109/13880200903578754",
language = "English (US)",
volume = "48",
pages = "1198--1206",
journal = "Pharmaceutical Biology",
issn = "1388-0209",
publisher = "Informa Healthcare",
number = "11",

}

TY - JOUR

T1 - Wound healing properties of Indian propolis studied on excision wound-induced rats

AU - Iyyam Pillai, S.

AU - Palsamy, P.

AU - Subramanian, S.

AU - Kandaswamy, M.

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Context: In traditional medicine propolis is widely used for the treatment of various ailments including ulcer and wound healing. The phytochemical screening of Indian propolis indicates the presence of biologically active ingredients in appreciable amounts. In the absence of systematic evaluation of wound healing properties of Indian propolis in the literature, the present study was undertaken. Objective: The aim of this study was to evaluate the wound healing potential of Indian propolis on excision wounds induced in experimental rats. Materials and methods: Excision wounds were created in male Wistar rats and were treated with Indian propolis ointment (nitrofurazone was used as a reference drug - widely used for wound healing) for a period of 14 days. Control rats were treated with petroleum jelly. The parameters analyzed include wound contraction, hydroxyproline, hexosamine, uronic acid, total protein, DNA, and RNA. Results: Topical application of propolis ointment for 14 days significantly improved the wound contraction when compared to the control group of rats. The determination of hydroxyproline, hexosamine, uronic acid, DNA, RNA and protein levels in the wound matrix revealed the pro-healing effects of propolis. The results obtained were comparable with nitrofurazone. Discussion and conclusion: It appears that the ethanol extract of Indian propolis possesses significant pro-healing activity by accelerating the healing process at various phases of tissue repair. The presence of biologically active ingredients such as flavonoids, phenolic acids, terpenes, benzoic acids, amino acids and vitamins, etc. in Indian propolis may readily account for the observed prophylactic action of propolis in wound healing.

AB - Context: In traditional medicine propolis is widely used for the treatment of various ailments including ulcer and wound healing. The phytochemical screening of Indian propolis indicates the presence of biologically active ingredients in appreciable amounts. In the absence of systematic evaluation of wound healing properties of Indian propolis in the literature, the present study was undertaken. Objective: The aim of this study was to evaluate the wound healing potential of Indian propolis on excision wounds induced in experimental rats. Materials and methods: Excision wounds were created in male Wistar rats and were treated with Indian propolis ointment (nitrofurazone was used as a reference drug - widely used for wound healing) for a period of 14 days. Control rats were treated with petroleum jelly. The parameters analyzed include wound contraction, hydroxyproline, hexosamine, uronic acid, total protein, DNA, and RNA. Results: Topical application of propolis ointment for 14 days significantly improved the wound contraction when compared to the control group of rats. The determination of hydroxyproline, hexosamine, uronic acid, DNA, RNA and protein levels in the wound matrix revealed the pro-healing effects of propolis. The results obtained were comparable with nitrofurazone. Discussion and conclusion: It appears that the ethanol extract of Indian propolis possesses significant pro-healing activity by accelerating the healing process at various phases of tissue repair. The presence of biologically active ingredients such as flavonoids, phenolic acids, terpenes, benzoic acids, amino acids and vitamins, etc. in Indian propolis may readily account for the observed prophylactic action of propolis in wound healing.

KW - Excision wound

KW - Flavonoids

KW - Hydroxyproline

KW - Indian propolis

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=78049454632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049454632&partnerID=8YFLogxK

U2 - 10.3109/13880200903578754

DO - 10.3109/13880200903578754

M3 - Article

C2 - 20819020

AN - SCOPUS:78049454632

VL - 48

SP - 1198

EP - 1206

JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

SN - 1388-0209

IS - 11

ER -