Wide variation in DNA content among isolates of Trypanosoma brucei ssp.

Georgette D. Kanmogne, Mick Bailey, Wendy C. Gibson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The DNA contents of 18 Trypanosoma brucei ssp. stocks were compared using flow cytometry, karyotype analysis and quantitation of repetitive DNA by Southern blotting and hybridisation. The DNA contents of Type I T. b. gambiense stocks were lower than those of non-gambiense stocks, but both groups showed a wide range of variation in DNA content. Amongst T. b. gambiense stocks, Mabia at the lower end of the range had 14% less DNA than Dal 972 at the top of the range. Similarly, amongst non-gambiense stocks, 117R at the lower end of the range had 14% less DNA than LM55 at the top of the range. The T. b. gambiense stock Mabia had 29% less DNA than non-gambiense stock LM55. The DNA content of Type II T. b. gambiense clearly fell within the non-gambiense range. Karyotype analysis showed that all T. b. gambiense stocks had minichromosomes, albeit fewer than non-gambiense stocks. This result was verified by hybridisation with probes for satellite DNA and a telomere-specific repeat. Hybridisation with a probe for the β-tubulin genes also revealed an apparent reduction of gene copy number in T. b. gambiense relative to non-gambiense stocks. In conclusion, there is a wide range of variation in genome size in T. brucei ssp., with T. b. gambiense stocks at the lower end of the range. The reduction in genome size correlates with loss of repeated genes and non-coding sequences in T. b. gambiense stocks, and is not confined to chromosomes of a particular size.

Original languageEnglish (US)
Pages (from-to)75-87
Number of pages13
JournalActa Tropica
Volume63
Issue number2-3
DOIs
StatePublished - Feb 1 1997

Fingerprint

Trypanosoma brucei
Trypanosoma brucei brucei
DNA
Genome Size
Karyotype
Southern blotting
karyotyping
hybridization
Satellite DNA
gene dosage
satellite DNA
Gene Dosage
genome
telomeres
Telomere
Tubulin
Southern Blotting
tubulin
Genes
flow cytometry

Keywords

  • DNA content
  • Trypanosoma brucei gambiense
  • chromosome
  • molecular karyotype

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

Cite this

Wide variation in DNA content among isolates of Trypanosoma brucei ssp. / Kanmogne, Georgette D.; Bailey, Mick; Gibson, Wendy C.

In: Acta Tropica, Vol. 63, No. 2-3, 01.02.1997, p. 75-87.

Research output: Contribution to journalArticle

Kanmogne, Georgette D. ; Bailey, Mick ; Gibson, Wendy C. / Wide variation in DNA content among isolates of Trypanosoma brucei ssp. In: Acta Tropica. 1997 ; Vol. 63, No. 2-3. pp. 75-87.
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abstract = "The DNA contents of 18 Trypanosoma brucei ssp. stocks were compared using flow cytometry, karyotype analysis and quantitation of repetitive DNA by Southern blotting and hybridisation. The DNA contents of Type I T. b. gambiense stocks were lower than those of non-gambiense stocks, but both groups showed a wide range of variation in DNA content. Amongst T. b. gambiense stocks, Mabia at the lower end of the range had 14{\%} less DNA than Dal 972 at the top of the range. Similarly, amongst non-gambiense stocks, 117R at the lower end of the range had 14{\%} less DNA than LM55 at the top of the range. The T. b. gambiense stock Mabia had 29{\%} less DNA than non-gambiense stock LM55. The DNA content of Type II T. b. gambiense clearly fell within the non-gambiense range. Karyotype analysis showed that all T. b. gambiense stocks had minichromosomes, albeit fewer than non-gambiense stocks. This result was verified by hybridisation with probes for satellite DNA and a telomere-specific repeat. Hybridisation with a probe for the β-tubulin genes also revealed an apparent reduction of gene copy number in T. b. gambiense relative to non-gambiense stocks. In conclusion, there is a wide range of variation in genome size in T. brucei ssp., with T. b. gambiense stocks at the lower end of the range. The reduction in genome size correlates with loss of repeated genes and non-coding sequences in T. b. gambiense stocks, and is not confined to chromosomes of a particular size.",
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