Whole genomic sequence analysis of Bacillus infantis

Defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe

Chandirasegaran Massilamany, Akram Mohammed, John Dustin Loy, Tanya Purvis, Bharathi Krishnan, Rakesh H. Basavalingappa, Christy M. Kelley, Chittibabu Guda, Raul G Barletta, Etsuko Moriyama, Timothy P L Smith, N R Jayagopala Reddy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: We recently reported the identification of Bacillus sp. NRRL B-14911 that induces heart autoimmunity by generating cardiac-reactive T cells through molecular mimicry. This marine bacterium was originally isolated from the Gulf of Mexico, but no associations with human diseases were reported. Therefore, to characterize its biological and medical significance, we sought to determine and analyze the complete genome sequence of Bacillus sp. NRRL B-14911. Results: Based on the phylogenetic analysis of 16S ribosomal RNA (rRNA) genes, sequence analysis of the 16S-23S rDNA intergenic transcribed spacers, phenotypic microarray, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, we propose that this organism belongs to the species Bacillus infantis, previously shown to be associated with sepsis in a newborn child. Analysis of the complete genome of Bacillus sp. NRRL B-14911 revealed several virulence factors including adhesins, invasins, colonization factors, siderophores and transporters. Likewise, the bacterial genome encodes a wide range of methyl transferases, transporters, enzymatic and biochemical pathways, and insertion sequence elements that are distinct from other closely related bacilli. Conclusions: The complete genome sequence of Bacillus sp. NRRL B-14911 provided in this study may facilitate genetic manipulations to assess gene functions associated with bacterial survival and virulence. Additionally, this bacterium may serve as a useful tool to establish a disease model that permits systematic analysis of autoimmune events in various susceptible rodent strains.

Original languageEnglish (US)
Article number511
JournalBMC genomics
Volume17
DOIs
StatePublished - Aug 22 2016

Fingerprint

Bacillus
Sequence Analysis
Genome
Gulf of Mexico
Molecular Mimicry
Bacteria
Bacterial Genomes
Siderophores
DNA Transposable Elements
Virulence Factors
Transferases
Ribosomal DNA
Autoimmunity
Genes
Virulence
Rodentia
Mass Spectrometry
Sepsis
Lasers
Newborn Infant

Keywords

  • Bacillus sp. NRRL B-14911
  • Genome
  • Heart

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Whole genomic sequence analysis of Bacillus infantis : Defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe. / Massilamany, Chandirasegaran; Mohammed, Akram; Loy, John Dustin; Purvis, Tanya; Krishnan, Bharathi; Basavalingappa, Rakesh H.; Kelley, Christy M.; Guda, Chittibabu; Barletta, Raul G; Moriyama, Etsuko; Smith, Timothy P L; Reddy, N R Jayagopala.

In: BMC genomics, Vol. 17, 511, 22.08.2016.

Research output: Contribution to journalArticle

Massilamany, Chandirasegaran ; Mohammed, Akram ; Loy, John Dustin ; Purvis, Tanya ; Krishnan, Bharathi ; Basavalingappa, Rakesh H. ; Kelley, Christy M. ; Guda, Chittibabu ; Barletta, Raul G ; Moriyama, Etsuko ; Smith, Timothy P L ; Reddy, N R Jayagopala. / Whole genomic sequence analysis of Bacillus infantis : Defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe. In: BMC genomics. 2016 ; Vol. 17.
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AU - Loy, John Dustin

AU - Purvis, Tanya

AU - Krishnan, Bharathi

AU - Basavalingappa, Rakesh H.

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