Wheat streak mosaic virus lacking helper component-proteinase is competent to produce disease synergism in double infections with Maize chlorotic mottle virus

Drake C. Stenger, Brock A. Young, Feng Qu, T. Jack Morris, Roy French

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The tritimovirus Wheat streak mosaic virus (WSMV) and the machlomovirus Maize chlorotic mottle virus (MCMV) each cause systemic chlorosis in infected maize plants. Infection of maize with both viruses produces corn lethal necrosis disease (CLND). Here, we report that complete deletion of the WSMV helper component-proteinase (HC-Pro) coding region had no effect on induction of CLND symptoms following coinoculation of maize with WSMV and MCMV. We further demonstrated that elevation of virus titers in double infections, relative to single infections, also was independent of WSMV HC-Pro. Thus, unlike potyvirus HC-Pro, WSMV HC-Pro was dispensable for disease synergism. Because disease synergism involving potyviruses requires HC-Promediated suppression of posttranscriptional gene silencing (PTGS), we hypothesized that WSMV HC-Pro may not be a suppressor of PTGS. Indeed, WSMV HC-Pro did not suppress PTGS of a green fluorescent protein (GFP) transgene in an Agrobacterium-mediated coinfiltration assay in which potyvirus HC-Pro acted as a strong suppressor. Furthermore, coinfiltration with potyvirus HC-Pro, but not WSMV HC-Pro, resulted in elevated levels of the GFP target mRNA under conditions which trigger PTGS. Collectively, these results revealed significant differences in HC-Pro function among divergent genera of the family Potyviridae and suggest that the tritimovirus WSMV utilizes a gene other than HC-Pro to suppress PTGS and mediate synergistic interactions with unrelated viruses.

Original languageEnglish (US)
Pages (from-to)1213-1221
Number of pages9
JournalPhytopathology
Volume97
Issue number10
DOIs
StatePublished - Oct 1 2007

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Maize chlorotic mottle virus
Wheat streak mosaic virus
synergism
proteinases
RNA interference
infection
Potyvirus
Tritimovirus
corn
Potyviridae
lethal genes
green fluorescent protein
Machlomovirus
necrosis
viruses
chlorosis
viral load
Agrobacterium

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Wheat streak mosaic virus lacking helper component-proteinase is competent to produce disease synergism in double infections with Maize chlorotic mottle virus. / Stenger, Drake C.; Young, Brock A.; Qu, Feng; Jack Morris, T.; French, Roy.

In: Phytopathology, Vol. 97, No. 10, 01.10.2007, p. 1213-1221.

Research output: Contribution to journalArticle

Stenger, Drake C. ; Young, Brock A. ; Qu, Feng ; Jack Morris, T. ; French, Roy. / Wheat streak mosaic virus lacking helper component-proteinase is competent to produce disease synergism in double infections with Maize chlorotic mottle virus. In: Phytopathology. 2007 ; Vol. 97, No. 10. pp. 1213-1221.
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