Water-Soluble Block Polycations as Carriers for OligonucleotiDe Delivery

Alexander V. Kabanov, Sergey V. Vinogradov, Yulia G. Suzdaltseva, Valery Yu Alakhov

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

Water-soluble, block copolymeric carriers consisting of polyoxyethylene (PEO) and polyspermine (PS) chains have been developed for the delivery of antisense oligonucleotides (oligo) into the target cells. These copolymers spontaneously form complexes with oligos in aqueous solutions. The PS block electrostatically binds to the oligo, and as a result, the stability of the oligo is increased. Similarly, the polar PEO block provides for the aqueous solubility of the complex. This paper (i) reports the synthesis of the diblock PEO-PS copolymer and (ii) evaluates the effects of the complexes formed between this copolymer and phosphodiester oligo, complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5, on the reproduction of this virus in Vero cells. Infectious titer data 22 and 39 h post infection indicates that the copolymer-oligo complex inhibits the reproduction of the virus beyond the detection limit. Conversely, the free oligo inhibits the reproduction of the virus only 22 h postinfection, while 39 h postinfection significant virus titers are observed. The results of this study suggest that the copolymeric complex increases the sequencespecific inhibition effect of oligo on the virus reproduction.

Original languageEnglish (US)
Pages (from-to)639-643
Number of pages5
JournalBioconjugate Chemistry
Volume6
Issue number6
DOIs
StatePublished - Jan 1 1995

Fingerprint

Oligonucleotides
Viruses
Reproduction
Water
Copolymers
Polyethylene oxides
Vero Cells
Antisense Oligonucleotides
RNA Precursors
Human Herpesvirus 1
Viral Load
Solubility
Limit of Detection
polycations
Polyethylene glycols
Infection

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Kabanov, A. V., Vinogradov, S. V., Suzdaltseva, Y. G., & Alakhov, V. Y. (1995). Water-Soluble Block Polycations as Carriers for OligonucleotiDe Delivery. Bioconjugate Chemistry, 6(6), 639-643. https://doi.org/10.1021/bc00036a001

Water-Soluble Block Polycations as Carriers for OligonucleotiDe Delivery. / Kabanov, Alexander V.; Vinogradov, Sergey V.; Suzdaltseva, Yulia G.; Alakhov, Valery Yu.

In: Bioconjugate Chemistry, Vol. 6, No. 6, 01.01.1995, p. 639-643.

Research output: Contribution to journalArticle

Kabanov, AV, Vinogradov, SV, Suzdaltseva, YG & Alakhov, VY 1995, 'Water-Soluble Block Polycations as Carriers for OligonucleotiDe Delivery', Bioconjugate Chemistry, vol. 6, no. 6, pp. 639-643. https://doi.org/10.1021/bc00036a001
Kabanov, Alexander V. ; Vinogradov, Sergey V. ; Suzdaltseva, Yulia G. ; Alakhov, Valery Yu. / Water-Soluble Block Polycations as Carriers for OligonucleotiDe Delivery. In: Bioconjugate Chemistry. 1995 ; Vol. 6, No. 6. pp. 639-643.
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