Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences

Gregory A. Golden, Todd A Wyatt, Debra Romberger, Daniel Reiff, Michael McCaskill, Christopher Bauer, Angela M. Gleason, Jill A Poole

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)2D3. Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalJournal of Biochemical and Molecular Toxicology
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Dust
Vitamin D
Neutrophils
Nutrition
Chemokines
Epithelial Cells
Diet
Lung
Interleukin-8
Cell culture
Gene expression
Inhalation
Monocytes
Animals
Blood
Swine
Animal Models
Cell Culture Techniques
Gene Expression

Keywords

  • Epithelial
  • Farm
  • Lung
  • Neutrophil
  • Organic dust
  • TLR
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences. / Golden, Gregory A.; Wyatt, Todd A; Romberger, Debra; Reiff, Daniel; McCaskill, Michael; Bauer, Christopher; Gleason, Angela M.; Poole, Jill A.

In: Journal of Biochemical and Molecular Toxicology, Vol. 27, No. 1, 01.01.2013, p. 77-86.

Research output: Contribution to journalArticle

Golden, Gregory A. ; Wyatt, Todd A ; Romberger, Debra ; Reiff, Daniel ; McCaskill, Michael ; Bauer, Christopher ; Gleason, Angela M. ; Poole, Jill A. / Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences. In: Journal of Biochemical and Molecular Toxicology. 2013 ; Vol. 27, No. 1. pp. 77-86.
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