Virus infection of culturable Chlorella-like algae and development of a plaque assay

James L. Van Etten, Dwight E. Burbank, Daniel Kuczmarski, Russel H. Meints

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus.

Original languageEnglish (US)
Pages (from-to)994-996
Number of pages3
JournalScience
Volume219
Issue number4587
DOIs
Publication statusPublished - Jan 1 1983

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this