Virus infection of culturable Chlorella-like algae and development of a plaque assay

James L. Van Etten, Dwight E. Burbank, Daniel Kuczmarski, Russel H. Meints

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus.

Original languageEnglish (US)
Pages (from-to)994-996
Number of pages3
JournalScience
Volume219
Issue number4587
DOIs
StatePublished - Jan 1 1983

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Chlorella
Virus Diseases
Viruses
Phycodnaviridae
Hydra
Paramecium
DNA Restriction Enzymes
Viral DNA
Invertebrates
DNA

ASJC Scopus subject areas

  • General

Cite this

Virus infection of culturable Chlorella-like algae and development of a plaque assay. / Van Etten, James L.; Burbank, Dwight E.; Kuczmarski, Daniel; Meints, Russel H.

In: Science, Vol. 219, No. 4587, 01.01.1983, p. 994-996.

Research output: Contribution to journalArticle

Van Etten, James L. ; Burbank, Dwight E. ; Kuczmarski, Daniel ; Meints, Russel H. / Virus infection of culturable Chlorella-like algae and development of a plaque assay. In: Science. 1983 ; Vol. 219, No. 4587. pp. 994-996.
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