Virus-host interactions: Insights from the replication cycle of the large Paramecium bursaria chlorella virus

Elad Milrot, Yael Mutsafi, Yael Fridmann-Sirkis, Eyal Shimoni, Katya Rechav, James R. Gurnon, James L. Van Etten, Abraham Minsky

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The increasing interest in cytoplasmic factories generated by eukaryotic-infecting viruses stems from the realization that these highly ordered assemblies may contribute fundamental novel insights to the functional significance of order in cellular biology. Here, we report the formation process and structural features of the cytoplasmic factories of the large dsDNA virus Paramecium bursaria chlorella virus 1 (PBCV-1). By combining diverse imaging techniques, including scanning transmission electron microscopy tomography and focused ion beam technologies, we show that the architecture and mode of formation of PBCV-1 factories are significantly different from those generated by their evolutionary relatives Vaccinia and Mimivirus. Specifically, PBCV-1 factories consist of a network of single membrane bilayers acting as capsid templates in the central region, and viral genomes spread throughout the host cytoplasm but excluded from the membrane-containing sites. In sharp contrast, factories generated by Mimivirus have viral genomes in their core, with membrane biogenesis region located at their periphery. Yet, all viral factories appear to share structural features that are essential for their function. In addition, our studies support the notion that PBCV-1 infection, which was recently reported to result in significant pathological outcomes in humans and mice, proceeds through a bacteriophage-like infection pathway.

Original languageEnglish (US)
Pages (from-to)3-16
Number of pages14
JournalCellular Microbiology
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Paramecium
Chlorella
Mimiviridae
Viruses
Viral Genome
Membranes
Electron Microscope Tomography
Vaccinia
Capsid
Virus Diseases
Bacteriophages
Cell Biology
Cytoplasm
Ions
Technology
Infection

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Milrot, E., Mutsafi, Y., Fridmann-Sirkis, Y., Shimoni, E., Rechav, K., Gurnon, J. R., ... Minsky, A. (2016). Virus-host interactions: Insights from the replication cycle of the large Paramecium bursaria chlorella virus. Cellular Microbiology, 18(1), 3-16. https://doi.org/10.1111/cmi.12486

Virus-host interactions : Insights from the replication cycle of the large Paramecium bursaria chlorella virus. / Milrot, Elad; Mutsafi, Yael; Fridmann-Sirkis, Yael; Shimoni, Eyal; Rechav, Katya; Gurnon, James R.; Van Etten, James L.; Minsky, Abraham.

In: Cellular Microbiology, Vol. 18, No. 1, 01.01.2016, p. 3-16.

Research output: Contribution to journalArticle

Milrot, E, Mutsafi, Y, Fridmann-Sirkis, Y, Shimoni, E, Rechav, K, Gurnon, JR, Van Etten, JL & Minsky, A 2016, 'Virus-host interactions: Insights from the replication cycle of the large Paramecium bursaria chlorella virus', Cellular Microbiology, vol. 18, no. 1, pp. 3-16. https://doi.org/10.1111/cmi.12486
Milrot, Elad ; Mutsafi, Yael ; Fridmann-Sirkis, Yael ; Shimoni, Eyal ; Rechav, Katya ; Gurnon, James R. ; Van Etten, James L. ; Minsky, Abraham. / Virus-host interactions : Insights from the replication cycle of the large Paramecium bursaria chlorella virus. In: Cellular Microbiology. 2016 ; Vol. 18, No. 1. pp. 3-16.
@article{28c68688d39f404e9e80f4b8771457c0,
title = "Virus-host interactions: Insights from the replication cycle of the large Paramecium bursaria chlorella virus",
abstract = "The increasing interest in cytoplasmic factories generated by eukaryotic-infecting viruses stems from the realization that these highly ordered assemblies may contribute fundamental novel insights to the functional significance of order in cellular biology. Here, we report the formation process and structural features of the cytoplasmic factories of the large dsDNA virus Paramecium bursaria chlorella virus 1 (PBCV-1). By combining diverse imaging techniques, including scanning transmission electron microscopy tomography and focused ion beam technologies, we show that the architecture and mode of formation of PBCV-1 factories are significantly different from those generated by their evolutionary relatives Vaccinia and Mimivirus. Specifically, PBCV-1 factories consist of a network of single membrane bilayers acting as capsid templates in the central region, and viral genomes spread throughout the host cytoplasm but excluded from the membrane-containing sites. In sharp contrast, factories generated by Mimivirus have viral genomes in their core, with membrane biogenesis region located at their periphery. Yet, all viral factories appear to share structural features that are essential for their function. In addition, our studies support the notion that PBCV-1 infection, which was recently reported to result in significant pathological outcomes in humans and mice, proceeds through a bacteriophage-like infection pathway.",
author = "Elad Milrot and Yael Mutsafi and Yael Fridmann-Sirkis and Eyal Shimoni and Katya Rechav and Gurnon, {James R.} and {Van Etten}, {James L.} and Abraham Minsky",
year = "2016",
month = "1",
day = "1",
doi = "10.1111/cmi.12486",
language = "English (US)",
volume = "18",
pages = "3--16",
journal = "Cellular Microbiology",
issn = "1462-5814",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Virus-host interactions

T2 - Insights from the replication cycle of the large Paramecium bursaria chlorella virus

AU - Milrot, Elad

AU - Mutsafi, Yael

AU - Fridmann-Sirkis, Yael

AU - Shimoni, Eyal

AU - Rechav, Katya

AU - Gurnon, James R.

AU - Van Etten, James L.

AU - Minsky, Abraham

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The increasing interest in cytoplasmic factories generated by eukaryotic-infecting viruses stems from the realization that these highly ordered assemblies may contribute fundamental novel insights to the functional significance of order in cellular biology. Here, we report the formation process and structural features of the cytoplasmic factories of the large dsDNA virus Paramecium bursaria chlorella virus 1 (PBCV-1). By combining diverse imaging techniques, including scanning transmission electron microscopy tomography and focused ion beam technologies, we show that the architecture and mode of formation of PBCV-1 factories are significantly different from those generated by their evolutionary relatives Vaccinia and Mimivirus. Specifically, PBCV-1 factories consist of a network of single membrane bilayers acting as capsid templates in the central region, and viral genomes spread throughout the host cytoplasm but excluded from the membrane-containing sites. In sharp contrast, factories generated by Mimivirus have viral genomes in their core, with membrane biogenesis region located at their periphery. Yet, all viral factories appear to share structural features that are essential for their function. In addition, our studies support the notion that PBCV-1 infection, which was recently reported to result in significant pathological outcomes in humans and mice, proceeds through a bacteriophage-like infection pathway.

AB - The increasing interest in cytoplasmic factories generated by eukaryotic-infecting viruses stems from the realization that these highly ordered assemblies may contribute fundamental novel insights to the functional significance of order in cellular biology. Here, we report the formation process and structural features of the cytoplasmic factories of the large dsDNA virus Paramecium bursaria chlorella virus 1 (PBCV-1). By combining diverse imaging techniques, including scanning transmission electron microscopy tomography and focused ion beam technologies, we show that the architecture and mode of formation of PBCV-1 factories are significantly different from those generated by their evolutionary relatives Vaccinia and Mimivirus. Specifically, PBCV-1 factories consist of a network of single membrane bilayers acting as capsid templates in the central region, and viral genomes spread throughout the host cytoplasm but excluded from the membrane-containing sites. In sharp contrast, factories generated by Mimivirus have viral genomes in their core, with membrane biogenesis region located at their periphery. Yet, all viral factories appear to share structural features that are essential for their function. In addition, our studies support the notion that PBCV-1 infection, which was recently reported to result in significant pathological outcomes in humans and mice, proceeds through a bacteriophage-like infection pathway.

UR - http://www.scopus.com/inward/record.url?scp=84940706666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940706666&partnerID=8YFLogxK

U2 - 10.1111/cmi.12486

DO - 10.1111/cmi.12486

M3 - Article

C2 - 26248343

AN - SCOPUS:84940706666

VL - 18

SP - 3

EP - 16

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5814

IS - 1

ER -