Virus detection by nucleic acid hybridization

Examination of normal and ALS tissues for the presence of poliovirus

D. E. Kohne, C. J. Gibbs, L. White, Steven M Tracy, W. Meinke, R. A. Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A nucleic acid hybridization assay was developed as a sensitive assay for the presence of poliovirus RNA in human tissue. The assay could detect the presence of an average of one poliovirus per 200 cells. A method for determining the extent of degradtion of the tissue RNA was developed and used to show that a significant fraction of human central nervous system (CNS) autopsy material contains highly degraded RNA which is unsuitable for hybridization studies. A total of 15 different control and amyotrophic lateral sclerosis tissues were assayed for the presence of poliovirus-like RNA. Virus RNA was detected in one of the control tissues and in none of the ALS tissues.

Original languageEnglish (US)
Pages (from-to)223-233
Number of pages11
JournalUnknown Journal
Volume56
Issue number2
DOIs
StatePublished - Jan 1 1981

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Nucleic Acid Hybridization
Poliovirus
Viruses
RNA
RNA Viruses
Amyotrophic Lateral Sclerosis
Autopsy
Central Nervous System

ASJC Scopus subject areas

  • Virology

Cite this

Virus detection by nucleic acid hybridization : Examination of normal and ALS tissues for the presence of poliovirus. / Kohne, D. E.; Gibbs, C. J.; White, L.; Tracy, Steven M; Meinke, W.; Smith, R. A.

In: Unknown Journal, Vol. 56, No. 2, 01.01.1981, p. 223-233.

Research output: Contribution to journalArticle

Kohne, D. E. ; Gibbs, C. J. ; White, L. ; Tracy, Steven M ; Meinke, W. ; Smith, R. A. / Virus detection by nucleic acid hybridization : Examination of normal and ALS tissues for the presence of poliovirus. In: Unknown Journal. 1981 ; Vol. 56, No. 2. pp. 223-233.
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