Viral trans-factor independent replication of human papillomavirus genomes

Daraporn Pittayakhajonwut, Peter C Angeletti

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background. Papillomaviruses (PVs) establish a persistent infection in the proliferating basal cells of the epithelium. The viral genome is replicated and maintained as a low-copy nuclear plasmid in basal keratinocytes. Bovine and human papillomaviruses (BPV and HPV) are known to utilize two viral proteins; E1, a DNA helicase, and E2, a transcription factor, which have been considered essential for viral DNA replication. However, growing evidence suggests that E1 and E2 are not entirely essential for stable replication of HPV. Results. Here we report that multiple HPV16 mutants, lacking either or both E1 and E2 open reading frame (ORFs) and the long control region (LCR), still support extrachromosomal replication. Our data clearly indicate that HPV16 has a mode of replication, independent of viral trans-factors, E1 and E2, which is achieved by origin activity located outside of the LCR.

Original languageEnglish (US)
Article number123
JournalVirology Journal
Volume7
DOIs
StatePublished - Jun 14 2010

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DNA Helicases
Viral Genome
Viral DNA
Viral Proteins
Human Genome
DNA Replication
Keratinocytes
Open Reading Frames
Plasmids
Transcription Factors
Epithelium
Infection

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Viral trans-factor independent replication of human papillomavirus genomes. / Pittayakhajonwut, Daraporn; Angeletti, Peter C.

In: Virology Journal, Vol. 7, 123, 14.06.2010.

Research output: Contribution to journalArticle

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