Coxsackievirus B3 infection has been associated with numerous human ailments including myocarditis, dilated cardiomyopathy, diabetes mellitus, and encephalomeningitis. Using the murine model we have shown by in situ hybridization (ISH) the localization of viral RNA in multiple tissues early post-infection. In this study we examined the geographical relationship between viral RNA (ISH) and cellular injury (terminal deoxynucleotidyl transferase-mediated in situ end labeling (TUNEL)) in multiple organs in myocarditissusceptible A/J and BALB/c mice and myocarditis-resistant C57BL/6J mice days 1 through 7 post-infection. Viral RNA localization and cellular injury were compared on contiguous sections. All organs positive for viral RNA were TUNEL positive for necrotic and/or apoptotic cellular injury in all murine strains. Organs with exclusive apoptotic death include spleen, thymus, lymph nodes, and central nervous system. Organs with a combination of necrotic and apoptotic mechanisms of cell death include heart, liver, pancreas, kidney, and salivary glands. Injury begins very ealy post-infection (day 2) during the period of initial viral RNA dissemination and localization in target organs, and continues throughout the duration of the study period. CVB3 induces direct cytolytic/necrotic injury and also operates through apoptotic mechanisms early following infection of multiple cell types. The significance of the distinct patterns of cell death and different mechanisms no doubt rests with target organ sequelae.
|Original language||English (US)|
|Publication status||Published - Dec 1 1997|
ASJC Scopus subject areas
- Molecular Biology