Very low ethanol concentrations affect the viability and growth recovery in post-stationary-phase Staphylococcus aureus populations

Indranil Chatterjee, Greg A Somerville, Christine Heilmann, Hans Georg Sahl, Hans H. Maurer, Mathias Herrmann

Research output: Contribution to journalArticle

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Abstract

Pharmaceuticals, culture media used for in vitro diagnostics and research, human body fluids, and environments can retain very low ethanol concentrations (VLEC) (≤0.1%, vol/vol). In contrast to the well-established effects of elevated ethanol concentrations on bacteria, little is known about the consequences of exposure to VLEC. We supplemented growth media for Staphylococcus aureus strain DSM20231 with VLEC (VLEC+ conditions) and determined ultramorphology, growth, and viability compared to those with unsupplemented media (VLEC conditions) for prolonged culture times (up to 8 days). VLEC+-grown late-stationary-phase S. aureus displayed extensive alterations of cell integrity as shown by scanning electron microscopy. Surprisingly, while ethanol in the medium was completely metabolized during exponential phase, a profound delay of S. aureus post-stationary-phase recovery (>48 h) was observed. Concomitantly, under VLEC+ conditions, the concentration of acetate in the culture medium remained elevated while that of ammonia was reduced, contributing to an acidic culture medium and suggesting decreased amino acid catabolism. Interestingly, amino acid depletion was not uniformly affected: under VLEC+ conditions, glutamic acid, ornithine, and proline remained in the culture medium while the uptake of other amino acids was not affected. Supplementation with arginine, but not with other amino acids, was able to restore post-stationary-phase growth and viability. Taken together, these data demonstrate that VLEC have profound effects on the recovery of S. aureus even after ethanol depletion and delay the transition from primary to secondary metabolite catabolism. These data also suggest that the concentration of ethanol needed for bacteriostatic control of S. aureus is lower than that previously reported.

Original languageEnglish (US)
Pages (from-to)2627-2636
Number of pages10
JournalApplied and environmental microbiology
Volume72
Issue number4
DOIs
StatePublished - Apr 1 2006

Fingerprint

Staphylococcus aureus
ethanol
viability
Ethanol
Growth
Population
culture media
Culture Media
amino acid
Amino Acids
catabolism
amino acids
body fluid
Ornithine
ornithine
body fluids
amino acid metabolism
secondary metabolite
Body Fluids
glutamic acid

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Very low ethanol concentrations affect the viability and growth recovery in post-stationary-phase Staphylococcus aureus populations. / Chatterjee, Indranil; Somerville, Greg A; Heilmann, Christine; Sahl, Hans Georg; Maurer, Hans H.; Herrmann, Mathias.

In: Applied and environmental microbiology, Vol. 72, No. 4, 01.04.2006, p. 2627-2636.

Research output: Contribution to journalArticle

Chatterjee, Indranil ; Somerville, Greg A ; Heilmann, Christine ; Sahl, Hans Georg ; Maurer, Hans H. ; Herrmann, Mathias. / Very low ethanol concentrations affect the viability and growth recovery in post-stationary-phase Staphylococcus aureus populations. In: Applied and environmental microbiology. 2006 ; Vol. 72, No. 4. pp. 2627-2636.
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