Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis

François Roudier, Lionel Gissot, Frédéric Beaudoin, Richard Haslam, Louise Michaelson, Jessica Marion, Diana Molino, Amparo Lima, Liên Bach, Halima Morin, Frédérique Tellier, Jean Christophe Palauqui, Yannick Bellec, Charlotte Renne, Martine Miquel, Marco DaCosta, Julien Vignard, Christine Rochat, Jonathan E Markham, Patrick Moreau & 2 others Johnathan Napier, Jean Denis Faure

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Very-long-chain fatty acids (VLCFAs) are essential for many aspects of plant development and necessary for the synthesis of seed storage triacylglycerols, epicuticular waxes, and sphingolipids. Identification of the acetyl-CoA carboxylase PASTICCINO3 and the 3-hydroxy acyl-CoA dehydratase PASTICCINO2 revealed that VLCFAs are important for cell proliferation and tissue patterning. Here, we show that the immunophilin PASTICCINO1 (PAS1) is also required for VLCFA synthesis. Impairment of PAS1 function results in reduction of VLCFA levels that particularly affects the composition of sphingolipids, known to be important for cell polarity in animals. Moreover, PAS1 associates with several enzymes of the VLCFA elongase complex in the endoplasmic reticulum. The pas1 mutants are deficient in lateral root formation and are characterized by an abnormal patterning of the embryo apex, which leads to defective cotyledon organogenesis. Our data indicate that in both tissues, defective organogenesis is associated with the mistargeting of the auxin efflux carrier PIN FORMED1 in specific cells, resulting in local alteration of polar auxin distribution. Furthermore, we show that exogenous VLCFAs rescue lateral root organogenesis and polar auxin distribution, indicating their direct involvement in these processes. Based on these data, we propose that PAS1 acts as a molecular scaffold for the fatty acid elongase complex in the endoplasmic reticulum and that the resulting VLCFAs are required for polar auxin transport and tissue patterning during plant development.

Original languageEnglish (US)
Pages (from-to)364-375
Number of pages12
JournalPlant Cell
Volume22
Issue number2
DOIs
StatePublished - Jan 1 2010

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very long chain fatty acids
Indoleacetic Acids
Arabidopsis
auxins
Fatty Acids
Organogenesis
Sphingolipids
Plant Development
organogenesis
Endoplasmic Reticulum
sphingolipids
Immunophilins
Hydro-Lyases
endoplasmic reticulum
Acetyl-CoA Carboxylase
plant development
Cell Polarity
Acyl Coenzyme A
Essential Fatty Acids
Cotyledon

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Roudier, F., Gissot, L., Beaudoin, F., Haslam, R., Michaelson, L., Marion, J., ... Faure, J. D. (2010). Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis. Plant Cell, 22(2), 364-375. https://doi.org/10.1105/tpc.109.071209

Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis. / Roudier, François; Gissot, Lionel; Beaudoin, Frédéric; Haslam, Richard; Michaelson, Louise; Marion, Jessica; Molino, Diana; Lima, Amparo; Bach, Liên; Morin, Halima; Tellier, Frédérique; Palauqui, Jean Christophe; Bellec, Yannick; Renne, Charlotte; Miquel, Martine; DaCosta, Marco; Vignard, Julien; Rochat, Christine; Markham, Jonathan E; Moreau, Patrick; Napier, Johnathan; Faure, Jean Denis.

In: Plant Cell, Vol. 22, No. 2, 01.01.2010, p. 364-375.

Research output: Contribution to journalArticle

Roudier, F, Gissot, L, Beaudoin, F, Haslam, R, Michaelson, L, Marion, J, Molino, D, Lima, A, Bach, L, Morin, H, Tellier, F, Palauqui, JC, Bellec, Y, Renne, C, Miquel, M, DaCosta, M, Vignard, J, Rochat, C, Markham, JE, Moreau, P, Napier, J & Faure, JD 2010, 'Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis', Plant Cell, vol. 22, no. 2, pp. 364-375. https://doi.org/10.1105/tpc.109.071209
Roudier, François ; Gissot, Lionel ; Beaudoin, Frédéric ; Haslam, Richard ; Michaelson, Louise ; Marion, Jessica ; Molino, Diana ; Lima, Amparo ; Bach, Liên ; Morin, Halima ; Tellier, Frédérique ; Palauqui, Jean Christophe ; Bellec, Yannick ; Renne, Charlotte ; Miquel, Martine ; DaCosta, Marco ; Vignard, Julien ; Rochat, Christine ; Markham, Jonathan E ; Moreau, Patrick ; Napier, Johnathan ; Faure, Jean Denis. / Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis. In: Plant Cell. 2010 ; Vol. 22, No. 2. pp. 364-375.
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AU - Tellier, Frédérique

AU - Palauqui, Jean Christophe

AU - Bellec, Yannick

AU - Renne, Charlotte

AU - Miquel, Martine

AU - DaCosta, Marco

AU - Vignard, Julien

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