V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation

Yui Hsi Wang, Zhixin Zhang, Peter D. Burrows, Hiromi Kubagawa, S. Louis Bridges, Harry W. Findley, Max D. Cooper

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The initial B-cell repertoire is generated by combinatorial immunoglobulin V(D)J gene segment rearrangements that occur in a preferential sequence. Because cellular proliferation occurs during the course of these rearrangement events, it has been proposed that intraclonal diversification occurs during this phase of B-cell development. An opportunity to examine this hypothesis directly was provided by the identification of a human acute lymphoblastic leukemic cell line that undergoes spontaneous differentiation from pro-B cell to the pre-B and B-cell stages with concomitant changes in the gene expression profile that normally occur during B-cell differentiation. After confirming the clonality of the progressively differentiating cells, an analysis of immunoglobulin genes and transcripts indicated that pro-B cell members marked by the same DJ rearrangement generated daughter B cells with multiple VH and VL gene segment rearrangements. These findings validate the principle of intraclonal V(D)J diversification during B-cell generation and define a manipulable model of human B-cell differentiation.

Original languageEnglish (US)
Pages (from-to)1030-1037
Number of pages8
JournalBlood
Volume101
Issue number3
DOIs
StatePublished - Feb 1 2003

Fingerprint

Cell Differentiation
B-Lymphocytes
Cells
B-Lymphoid Precursor Cells
Gene Rearrangement
Genes
Immunoglobulin Variable Region
Immunoglobulin D
Forensic Anthropology
Immunoglobulin Genes
Transcriptome
Cell Proliferation
Gene expression
Cell Line
Immunoglobulins

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Wang, Y. H., Zhang, Z., Burrows, P. D., Kubagawa, H., Bridges, S. L., Findley, H. W., & Cooper, M. D. (2003). V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation. Blood, 101(3), 1030-1037. https://doi.org/10.1182/blood-2002-06-1828

V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation. / Wang, Yui Hsi; Zhang, Zhixin; Burrows, Peter D.; Kubagawa, Hiromi; Bridges, S. Louis; Findley, Harry W.; Cooper, Max D.

In: Blood, Vol. 101, No. 3, 01.02.2003, p. 1030-1037.

Research output: Contribution to journalArticle

Wang, YH, Zhang, Z, Burrows, PD, Kubagawa, H, Bridges, SL, Findley, HW & Cooper, MD 2003, 'V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation', Blood, vol. 101, no. 3, pp. 1030-1037. https://doi.org/10.1182/blood-2002-06-1828
Wang YH, Zhang Z, Burrows PD, Kubagawa H, Bridges SL, Findley HW et al. V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation. Blood. 2003 Feb 1;101(3):1030-1037. https://doi.org/10.1182/blood-2002-06-1828
Wang, Yui Hsi ; Zhang, Zhixin ; Burrows, Peter D. ; Kubagawa, Hiromi ; Bridges, S. Louis ; Findley, Harry W. ; Cooper, Max D. / V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation. In: Blood. 2003 ; Vol. 101, No. 3. pp. 1030-1037.
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