VAV1-Cre mediated hematopoietic deletion of CBL and CBL-B leads to JMML-like aggressive early-neonatal myeloproliferative disease

Wei An, Bhopal C. Mohapatra, Neha Zutshi, Timothy A. Bielecki, Benjamin T. Goez, Haitao Luan, Fany Iseka, Insha Mushtaq, Matthew D. Storck, Vimla Band, Hamid Band

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

CBL and CBL-B ubiquitin ligases play key roles in hematopoietic stem cell homeostasis and their aberrations are linked to leukemogenesis. Mutations of CBL, often genetically-inherited, are particularly common in Juvenile Myelomonocytic Leukemia (JMML), a disease that manifests early in children. JMML is fatal unless corrected by bone marrow transplant, which is effective in only half of the recipients, stressing the need for animal models that recapitulate the key clinical features of this disease. However, mouse models established so far only develop hematological malignancy in adult animals. Here, using VAV1-Cre-induced conditional CBL/CBL-B double knockout (DKO) in mice, we established an animal model that exhibits a neonatal myeloproliferative disease (MPD). VAV1-Cre induced DKO mice developed a strong hematological phenotype at postnatal day 10, including severe leukocytosis and hepatomegaly, bone marrow cell hypersensitivity to cytokines including GM-CSF, and rapidly-progressive disease and invariable lethality. Interestingly, leukemic stem cells were most highly enriched in neonatal liver rather than bone marrow, which, along with the spleen and thymus, were hypo-cellular. Nonetheless, transplantation assays showed that both DKO bone marrow and liver cells can initiate leukemic disease in the recipient mice with seeding of both spleen and bone marrow. Together, our results support the usefulness of the new hematopoietic-specific CBL/CBL-B double KO animal model to study JMML-related pathogenesis and to further understand the function of CBL family proteins in regulating fetal and neonatal hematopoiesis. To our knowledge, this is the first mouse model that exhibits neonatal MPD in infancy, by day 10 of postnatal life.

Original languageEnglish (US)
Pages (from-to)59006-59016
Number of pages11
JournalOncotarget
Volume7
Issue number37
DOIs
StatePublished - 2016

Fingerprint

Infant, Newborn, Diseases
Juvenile Myelomonocytic Leukemia
Animal Models
Bone Marrow
Knockout Mice
Bone Marrow Cells
Spleen
Hepatomegaly
Liver
Leukocytosis
Hematopoiesis
Hematologic Neoplasms
Granulocyte-Macrophage Colony-Stimulating Factor
Ligases
Hematopoietic Stem Cells
Ubiquitin
Thymus Gland
Hypersensitivity
Homeostasis
Stem Cells

Keywords

  • CBL
  • HSC
  • JMML
  • Neonatal hematopoiesis
  • Ubiquitin ligase

ASJC Scopus subject areas

  • Oncology

Cite this

VAV1-Cre mediated hematopoietic deletion of CBL and CBL-B leads to JMML-like aggressive early-neonatal myeloproliferative disease. / An, Wei; Mohapatra, Bhopal C.; Zutshi, Neha; Bielecki, Timothy A.; Goez, Benjamin T.; Luan, Haitao; Iseka, Fany; Mushtaq, Insha; Storck, Matthew D.; Band, Vimla; Band, Hamid.

In: Oncotarget, Vol. 7, No. 37, 2016, p. 59006-59016.

Research output: Contribution to journalArticle

An, W, Mohapatra, BC, Zutshi, N, Bielecki, TA, Goez, BT, Luan, H, Iseka, F, Mushtaq, I, Storck, MD, Band, V & Band, H 2016, 'VAV1-Cre mediated hematopoietic deletion of CBL and CBL-B leads to JMML-like aggressive early-neonatal myeloproliferative disease', Oncotarget, vol. 7, no. 37, pp. 59006-59016. https://doi.org/10.18632/oncotarget.10638
An, Wei ; Mohapatra, Bhopal C. ; Zutshi, Neha ; Bielecki, Timothy A. ; Goez, Benjamin T. ; Luan, Haitao ; Iseka, Fany ; Mushtaq, Insha ; Storck, Matthew D. ; Band, Vimla ; Band, Hamid. / VAV1-Cre mediated hematopoietic deletion of CBL and CBL-B leads to JMML-like aggressive early-neonatal myeloproliferative disease. In: Oncotarget. 2016 ; Vol. 7, No. 37. pp. 59006-59016.
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abstract = "CBL and CBL-B ubiquitin ligases play key roles in hematopoietic stem cell homeostasis and their aberrations are linked to leukemogenesis. Mutations of CBL, often genetically-inherited, are particularly common in Juvenile Myelomonocytic Leukemia (JMML), a disease that manifests early in children. JMML is fatal unless corrected by bone marrow transplant, which is effective in only half of the recipients, stressing the need for animal models that recapitulate the key clinical features of this disease. However, mouse models established so far only develop hematological malignancy in adult animals. Here, using VAV1-Cre-induced conditional CBL/CBL-B double knockout (DKO) in mice, we established an animal model that exhibits a neonatal myeloproliferative disease (MPD). VAV1-Cre induced DKO mice developed a strong hematological phenotype at postnatal day 10, including severe leukocytosis and hepatomegaly, bone marrow cell hypersensitivity to cytokines including GM-CSF, and rapidly-progressive disease and invariable lethality. Interestingly, leukemic stem cells were most highly enriched in neonatal liver rather than bone marrow, which, along with the spleen and thymus, were hypo-cellular. Nonetheless, transplantation assays showed that both DKO bone marrow and liver cells can initiate leukemic disease in the recipient mice with seeding of both spleen and bone marrow. Together, our results support the usefulness of the new hematopoietic-specific CBL/CBL-B double KO animal model to study JMML-related pathogenesis and to further understand the function of CBL family proteins in regulating fetal and neonatal hematopoiesis. To our knowledge, this is the first mouse model that exhibits neonatal MPD in infancy, by day 10 of postnatal life.",
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