Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea

G. D. Phillips, A. M. Stone, B. D. Jones, J. C. Schultz, R. A. Whitehead, D. R. Knighton

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The object of this study was to test vascular endothelial- growth factor (VEGF) for angiogenic activity in the rabbit corneal assay. VEGF doses ranging from 20ng to 1000ng were incorporated into a slow release polymer and implanted into the avascular rabbit cornea. Capillary formation in the cornea was visually analyzed on a daily basis and examined with histology, transmission electron microscopy and scanning electron microscopy of vascular corrosion casts on days 2 and 7 post-implantation. VEGF implants (200ng to 1000ng) consistently stimulated angiogenesis. This neovascularization occurred in the absence of inflammation. We conclude that VEGF acts directly on endothelial cells, initiating and mediating the formation of capillaries.

Original languageEnglish (US)
Pages (from-to)961-965
Number of pages5
JournalIn Vivo
Volume8
Issue number6
StatePublished - Dec 1 1994

Fingerprint

Cornea
Vascular Endothelial Growth Factor A
Rabbits
Histology
Corrosion
Endothelial cells
Transmission Electron Microscopy
Electron Scanning Microscopy
Blood Vessels
Assays
Polymers
Endothelial Cells
Transmission electron microscopy
Inflammation
Scanning electron microscopy

Keywords

  • Angiogenesis
  • In vivo
  • Rabbit cornea
  • VEGF

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Phillips, G. D., Stone, A. M., Jones, B. D., Schultz, J. C., Whitehead, R. A., & Knighton, D. R. (1994). Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea. In Vivo, 8(6), 961-965.

Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea. / Phillips, G. D.; Stone, A. M.; Jones, B. D.; Schultz, J. C.; Whitehead, R. A.; Knighton, D. R.

In: In Vivo, Vol. 8, No. 6, 01.12.1994, p. 961-965.

Research output: Contribution to journalArticle

Phillips, GD, Stone, AM, Jones, BD, Schultz, JC, Whitehead, RA & Knighton, DR 1994, 'Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea', In Vivo, vol. 8, no. 6, pp. 961-965.
Phillips GD, Stone AM, Jones BD, Schultz JC, Whitehead RA, Knighton DR. Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea. In Vivo. 1994 Dec 1;8(6):961-965.
Phillips, G. D. ; Stone, A. M. ; Jones, B. D. ; Schultz, J. C. ; Whitehead, R. A. ; Knighton, D. R. / Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea. In: In Vivo. 1994 ; Vol. 8, No. 6. pp. 961-965.
@article{43928437044b4c208143ed4c3353b9ad,
title = "Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea",
abstract = "The object of this study was to test vascular endothelial- growth factor (VEGF) for angiogenic activity in the rabbit corneal assay. VEGF doses ranging from 20ng to 1000ng were incorporated into a slow release polymer and implanted into the avascular rabbit cornea. Capillary formation in the cornea was visually analyzed on a daily basis and examined with histology, transmission electron microscopy and scanning electron microscopy of vascular corrosion casts on days 2 and 7 post-implantation. VEGF implants (200ng to 1000ng) consistently stimulated angiogenesis. This neovascularization occurred in the absence of inflammation. We conclude that VEGF acts directly on endothelial cells, initiating and mediating the formation of capillaries.",
keywords = "Angiogenesis, In vivo, Rabbit cornea, VEGF",
author = "Phillips, {G. D.} and Stone, {A. M.} and Jones, {B. D.} and Schultz, {J. C.} and Whitehead, {R. A.} and Knighton, {D. R.}",
year = "1994",
month = "12",
day = "1",
language = "English (US)",
volume = "8",
pages = "961--965",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "6",

}

TY - JOUR

T1 - Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea

AU - Phillips, G. D.

AU - Stone, A. M.

AU - Jones, B. D.

AU - Schultz, J. C.

AU - Whitehead, R. A.

AU - Knighton, D. R.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - The object of this study was to test vascular endothelial- growth factor (VEGF) for angiogenic activity in the rabbit corneal assay. VEGF doses ranging from 20ng to 1000ng were incorporated into a slow release polymer and implanted into the avascular rabbit cornea. Capillary formation in the cornea was visually analyzed on a daily basis and examined with histology, transmission electron microscopy and scanning electron microscopy of vascular corrosion casts on days 2 and 7 post-implantation. VEGF implants (200ng to 1000ng) consistently stimulated angiogenesis. This neovascularization occurred in the absence of inflammation. We conclude that VEGF acts directly on endothelial cells, initiating and mediating the formation of capillaries.

AB - The object of this study was to test vascular endothelial- growth factor (VEGF) for angiogenic activity in the rabbit corneal assay. VEGF doses ranging from 20ng to 1000ng were incorporated into a slow release polymer and implanted into the avascular rabbit cornea. Capillary formation in the cornea was visually analyzed on a daily basis and examined with histology, transmission electron microscopy and scanning electron microscopy of vascular corrosion casts on days 2 and 7 post-implantation. VEGF implants (200ng to 1000ng) consistently stimulated angiogenesis. This neovascularization occurred in the absence of inflammation. We conclude that VEGF acts directly on endothelial cells, initiating and mediating the formation of capillaries.

KW - Angiogenesis

KW - In vivo

KW - Rabbit cornea

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=0028670958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028670958&partnerID=8YFLogxK

M3 - Article

C2 - 7539637

AN - SCOPUS:0028670958

VL - 8

SP - 961

EP - 965

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 6

ER -