VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus

Yukari Maezato, Amanda Daugherty, Karl Dana, Edith Soo, Charlotte Cooper, Sabrina Tachdjian, Robert M. Kelly, Paul Blum

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The phylum Crenarchaeota includes hyperthermophilic micro-organisms subjected to dynamic thermal conditions. Previous transcriptomic studies of Sulfolobus solfataricus identified vapBC6 as a heat-shock (HS)-inducible member of the Vap toxin-antitoxin gene family. In this study, the inactivation of the vapBC6 operon by targeted gene disruption produced two recessive phenotypes related to fitness, HS sensitivity and a heat-dependent reduction in the rate of growth. In-frame vapBC6 deletion mutants were analyzed to examine the respective roles of each protein. Since vapB6 transcript abundance was elevated in the vapC6 deletion, the VapC6 toxin appears to regulate abundance of its cognate antitoxin. In contrast, vapC6 transcript abundance was reduced in the vapB6 deletion. A putative intergenic terminator may underlie these observations by coordinating vapBC6 expression. As predicted by structural modeling, recombinant VapC6 produced using chaperone cosynthesis exhibited heat-dependent ribonucleolytic activity toward S. solfataricus total RNA. This activity could be blocked by addition of preheated recombinant VapB6. In vivo transcript targets were identified by assessing the relative expression of genes that naturally respond to thermal stress in VapBC6-deficient cells. Preferential increases were observed for dppB-1 and tetR, and preferential decreases were observed for rpoD and eIF2 gamma. Specific VapC6 ribonucleolytic action could also be demonstrated in vitro toward RNAs whose expression increased in the VapBC6-deficient strain during heat shock. These findings provide a biochemical mechanism and identify cellular targets underlying VapBC6-mediated control over microbial growth and survival at temperature extremes. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1381-1392
Number of pages12
JournalRNA
Volume17
Issue number7
DOIs
StatePublished - Jul 1 2011

Fingerprint

Sulfolobus solfataricus
Hot Temperature
Antitoxins
Shock
Crenarchaeota
RNA
Operon
Growth
Genes
Phenotype
Gene Expression
Temperature

Keywords

  • Antitoxin
  • Archaea
  • Heat shock
  • RNA degradation
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Maezato, Y., Daugherty, A., Dana, K., Soo, E., Cooper, C., Tachdjian, S., ... Blum, P. (2011). VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus. RNA, 17(7), 1381-1392. https://doi.org/10.1261/rna.2679911

VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus. / Maezato, Yukari; Daugherty, Amanda; Dana, Karl; Soo, Edith; Cooper, Charlotte; Tachdjian, Sabrina; Kelly, Robert M.; Blum, Paul.

In: RNA, Vol. 17, No. 7, 01.07.2011, p. 1381-1392.

Research output: Contribution to journalArticle

Maezato, Y, Daugherty, A, Dana, K, Soo, E, Cooper, C, Tachdjian, S, Kelly, RM & Blum, P 2011, 'VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus', RNA, vol. 17, no. 7, pp. 1381-1392. https://doi.org/10.1261/rna.2679911
Maezato Y, Daugherty A, Dana K, Soo E, Cooper C, Tachdjian S et al. VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus. RNA. 2011 Jul 1;17(7):1381-1392. https://doi.org/10.1261/rna.2679911
Maezato, Yukari ; Daugherty, Amanda ; Dana, Karl ; Soo, Edith ; Cooper, Charlotte ; Tachdjian, Sabrina ; Kelly, Robert M. ; Blum, Paul. / VapC6, a ribonucleolytic toxin regulates thermophilicity in the crenarchaeote Sulfolobus solfataricus. In: RNA. 2011 ; Vol. 17, No. 7. pp. 1381-1392.
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