Vancomycin serum protein binding determination by ultrafiltration

B. H. Ackerman, E. H. Taylor, K. M. Olsen, W. Abdel-Malak, A. A. Pappas

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Sixty-two serum concentrations were obtained from 12 infected patients enrolled in a vancomycin pharmacokinetic study. Both unbound and total serum vancomycin concentrations were measured using ultrafiltration and a commercial fluorescent polarization immunoassay. Ultrafiltrates were obtained by centrifugation at 1000 x g for ten minutes at room temperature and their assay indicated a range in protein binding from 7.9 to 71 percent. The mean protein binding (mean ± SD) was 41.95 ± 14.15 percent. No measurable adsorption of vancomycin onto the ultrafiltration membrane was noted. Orthogonal regression of unbound versus total vancomycin concentrations was described by the equation y = 0.597x-0.362 with a correlation coefficient of 0.948.

Original languageEnglish (US)
Pages (from-to)300-303
Number of pages4
JournalDrug Intelligence and Clinical Pharmacy
Volume22
Issue number4
DOIs
Publication statusPublished - Jan 1 1988

    Fingerprint

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Ackerman, B. H., Taylor, E. H., Olsen, K. M., Abdel-Malak, W., & Pappas, A. A. (1988). Vancomycin serum protein binding determination by ultrafiltration. Drug Intelligence and Clinical Pharmacy, 22(4), 300-303. https://doi.org/10.1177/106002808802200404