Value of monoclonal antibodies in the diagnosis of Hodgkin's disease in paraffin sections

Y. L. Ye, J. Linder

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Abstract

A comprehensive panel of monoclonal antibodies that mark R-S/H cell, T- and B-cell, monocyte/histocyte was tested in paraffin sections of 107 cases of Hodgkin's disease (HD) including 21 cases of lymphocyte predominance (LP) and 86 cases of Non-LP HD. Thirty cases each of peripheral T-cell lymphoma and B-cell lymphoma were also tested for comparison. R-S/H cells were not stained with T-cell marker (UCHL-1) or monocyte/histocyte marker (Mac387) in all of these cases of HD. The results showed presence of certain difference in the phenotype of LP from non-LP. The H+L type of R-S/H cells of LP often reacted with B-cell markers including L26, LN2, LN1 and MB2 (93.3%-100%), LCA (83.3%) and EMA (92.3%), but rarely with LeuM1,T mü 9, or BerH2. On the contrary, most of the R-S/H cells of non-LP reacted with LeuM1 (80%), T mu 9(84%), BerH2(65%) but not with B-cell markers, LCA or EMA. Our study suggests a B-cell (probably the follicular center cell) derivation for L+H type of R-S/H cells in LP. The fact that 1 case of LP in this group transformed to a large cell B-cell lymphoma also supports this consideration. PNA is a sensitive marker of R-S/H cells but is not a specific one, since PNA stains 43.3% of the peripheral T-cell lymphoma and 20% of the B-cell lymphomas. Our findings indicate that using a panel of antibodies which mark R-S/H cells, T- and B-cells in paraffin sections will be helpful in the diagnosis and subtyping of Hodgkin's disease.

Original languageEnglish (US)
Pages (from-to)40-43
Number of pages4
JournalZhonghua bing li xue za zhi Chinese journal of pathology
Volume21
Issue number1
StatePublished - Feb 1992

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Hodgkin Disease
Paraffin
Monoclonal Antibodies
B-Lymphocytes
Lymphocytes
B-Cell Lymphoma
Peripheral T-Cell Lymphoma
Monocytes
Coloring Agents
T-Lymphocytes
Phenotype
Antibodies

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Value of monoclonal antibodies in the diagnosis of Hodgkin's disease in paraffin sections",
abstract = "A comprehensive panel of monoclonal antibodies that mark R-S/H cell, T- and B-cell, monocyte/histocyte was tested in paraffin sections of 107 cases of Hodgkin's disease (HD) including 21 cases of lymphocyte predominance (LP) and 86 cases of Non-LP HD. Thirty cases each of peripheral T-cell lymphoma and B-cell lymphoma were also tested for comparison. R-S/H cells were not stained with T-cell marker (UCHL-1) or monocyte/histocyte marker (Mac387) in all of these cases of HD. The results showed presence of certain difference in the phenotype of LP from non-LP. The H+L type of R-S/H cells of LP often reacted with B-cell markers including L26, LN2, LN1 and MB2 (93.3{\%}-100{\%}), LCA (83.3{\%}) and EMA (92.3{\%}), but rarely with LeuM1,T m{\"u} 9, or BerH2. On the contrary, most of the R-S/H cells of non-LP reacted with LeuM1 (80{\%}), T mu 9(84{\%}), BerH2(65{\%}) but not with B-cell markers, LCA or EMA. Our study suggests a B-cell (probably the follicular center cell) derivation for L+H type of R-S/H cells in LP. The fact that 1 case of LP in this group transformed to a large cell B-cell lymphoma also supports this consideration. PNA is a sensitive marker of R-S/H cells but is not a specific one, since PNA stains 43.3{\%} of the peripheral T-cell lymphoma and 20{\%} of the B-cell lymphomas. Our findings indicate that using a panel of antibodies which mark R-S/H cells, T- and B-cells in paraffin sections will be helpful in the diagnosis and subtyping of Hodgkin's disease.",
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AU - Linder, J.

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