Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening

Channabasavaiah B Gurumurthy, Poonam S. Joshi, Scott G. Kurz, Masato Ohtsuka, Rolen M. Quadros, Donald W. Harms, K. C Kent Lloyd

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Marker assisted speed congenics technique is commonly used to facilitate backcrossing of mouse strains in nearly half the time it normally takes otherwise. Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation. Although there are well-defined panels of SSLP makers established between certain pairs of mice strains, they are incomplete for most strains. The availability of well-established marker sets for speed congenic screens would enable the scientific community to transfer mutations across strain backgrounds. In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models. The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens. Moreover, unlike newer single nucleotide polymorphism (SNP) array methods which require sophisticated equipment, the SSLP markers panel described here only uses PCR and agarose gel electrophoresis of amplified products; therefore it can be performed in most research laboratories.

Original languageEnglish (US)
Article number735845
JournalInternational Journal of Genomics
Volume2015
DOIs
StatePublished - Feb 28 2015

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Polymorphism
Screening
Polymerase Chain Reaction
Inbreeding
Agar Gel Electrophoresis
Single Nucleotide Polymorphism
Chromosomes
Genome
Equipment and Supplies
Mutation
Research laboratories
Electrophoresis
Research
Sepharose
Nucleotides
Genes
Gels
Availability

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Pharmaceutical Science

Cite this

Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening. / Gurumurthy, Channabasavaiah B; Joshi, Poonam S.; Kurz, Scott G.; Ohtsuka, Masato; Quadros, Rolen M.; Harms, Donald W.; Lloyd, K. C Kent.

In: International Journal of Genomics, Vol. 2015, 735845, 28.02.2015.

Research output: Contribution to journalArticle

Gurumurthy, Channabasavaiah B ; Joshi, Poonam S. ; Kurz, Scott G. ; Ohtsuka, Masato ; Quadros, Rolen M. ; Harms, Donald W. ; Lloyd, K. C Kent. / Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening. In: International Journal of Genomics. 2015 ; Vol. 2015.
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