Vacuole dynamics in the salivary glands of Drosophila melanogaster during prepupal development

Robert Farkaš, Denisa Beňová-Liszeková, Lucia Mentelová, Silvia Mahmood, Zuzana Ďatková, Milan Beňo, Ludmila Pečeňová, Otakar Raška, Jana Šmigová, Bruce A Chase, Ivan Raška, Bernard M. Mechler

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A central function of the Drosophila salivary glands (SGs), historically known for their polytene chromosomes, is to produce and then release during pupariation the secretory glue used to affix a newly formed puparium to a substrate. This essential event in the life history of Drosophila is regulated by the steroid hormone ecdysone in the late-larval period. Ecdysone triggers a cascade of sequential gene activation that leads to glue secretion and initiates the developmentally-regulated programmed cell death (PCD) of the larval salivary glands, which culminates 16 h after puparium formation (APF). We demonstrate here that, even after the larval salivary glands have completed what is perceived to be one of their major biological functions - glue secretion during pupariation - they remain dynamic and physiologically active up until the execution phase of PCD. We have used specific metabolic inhibitors and genetic tools, including mutations or transgenes for shi, Rab5, Rab11, vha55, vha68-2, vha36-1, syx1A, syx4, and Vps35 to characterize the dramatic series of cellular changes occurring in the SG cells between pupariation and 7-8 h APF. Early in the prepupal period, they are remarkably active in endocytosis, forming acidic vacuoles. Midway through the prepupal period, there is abundant late endosomal trafficking and vacuole growth, which is followed later by vacuole neutralization and disappearance via membrane consolidation. This work provides new insights into the function of Drosophila SGs during the early- to mid-prepupal period.

Original languageEnglish (US)
Pages (from-to)74-96
Number of pages23
JournalDevelopment Growth and Differentiation
Volume57
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Vacuoles
Salivary Glands
Drosophila melanogaster
Adhesives
Drosophila
Ecdysone
Cell Death
Polytene Chromosomes
Endocytosis
Transgenes
Transcriptional Activation
Steroids
Hormones
Mutation
Membranes
Growth

Keywords

  • Endosomal trafficking
  • Metamorphosis
  • Prepupal period
  • Salivary glands
  • Vacuolation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Farkaš, R., Beňová-Liszeková, D., Mentelová, L., Mahmood, S., Ďatková, Z., Beňo, M., ... Mechler, B. M. (2015). Vacuole dynamics in the salivary glands of Drosophila melanogaster during prepupal development. Development Growth and Differentiation, 57(1), 74-96. https://doi.org/10.1111/dgd.12193

Vacuole dynamics in the salivary glands of Drosophila melanogaster during prepupal development. / Farkaš, Robert; Beňová-Liszeková, Denisa; Mentelová, Lucia; Mahmood, Silvia; Ďatková, Zuzana; Beňo, Milan; Pečeňová, Ludmila; Raška, Otakar; Šmigová, Jana; Chase, Bruce A; Raška, Ivan; Mechler, Bernard M.

In: Development Growth and Differentiation, Vol. 57, No. 1, 01.01.2015, p. 74-96.

Research output: Contribution to journalArticle

Farkaš, R, Beňová-Liszeková, D, Mentelová, L, Mahmood, S, Ďatková, Z, Beňo, M, Pečeňová, L, Raška, O, Šmigová, J, Chase, BA, Raška, I & Mechler, BM 2015, 'Vacuole dynamics in the salivary glands of Drosophila melanogaster during prepupal development', Development Growth and Differentiation, vol. 57, no. 1, pp. 74-96. https://doi.org/10.1111/dgd.12193
Farkaš R, Beňová-Liszeková D, Mentelová L, Mahmood S, Ďatková Z, Beňo M et al. Vacuole dynamics in the salivary glands of Drosophila melanogaster during prepupal development. Development Growth and Differentiation. 2015 Jan 1;57(1):74-96. https://doi.org/10.1111/dgd.12193
Farkaš, Robert ; Beňová-Liszeková, Denisa ; Mentelová, Lucia ; Mahmood, Silvia ; Ďatková, Zuzana ; Beňo, Milan ; Pečeňová, Ludmila ; Raška, Otakar ; Šmigová, Jana ; Chase, Bruce A ; Raška, Ivan ; Mechler, Bernard M. / Vacuole dynamics in the salivary glands of Drosophila melanogaster during prepupal development. In: Development Growth and Differentiation. 2015 ; Vol. 57, No. 1. pp. 74-96.
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