Vγ2Vδ2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer

J. F. Bukowski, C. T. Morita, Y. Tanaka, B. R. Bloom, M. B. Brenner, Hamid Band

Research output: Contribution to journalArticle

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Abstract

The predominant subpopulation of γδ T cells in human peripheral blood expresses TCR V region genes Vγ2 paired with Vδ2. Previous studies have shown that these Vγ2Vδ2 T cells proliferate in response to Daudi Burkitt lymphoma cells, synthetic alkyl phosphate molecules including monoethylphosphate (MEP), and an Ag chemically similar to MEP purified from mycobacterial extracts of several species including Mycobacterium tuberculosis. This proliferation is polyclonal and determined by the TCR V gene. However, because these alkyl phosphate molecules are so distinct from conventional peptides and superantigens, we questioned whether these substances induce γδ T cell proliferation via TCR-dependent recognition. Here we report that transfection of TCR- Jurkat T cells with cDNA constructs encoding a Vγ2Vδ2 TCR enabled the transfectants to produce IL-2 in response to Daudi cells, mycobacterial extract, and MEP. The responses were dose dependent and Ag specific. These results demonstrate an essential role for the γδ TCR in Vγ2Vδ2 T cell mediated recognition of non-peptide Ags by human T cells and suggest a structural similarity or cross-reactivity between cellular and microbial Ags recognized by these γδ T cells.

Original languageEnglish (US)
Pages (from-to)998-1006
Number of pages9
JournalJournal of Immunology
Volume154
Issue number3
StatePublished - Jan 1 1995
Externally publishedYes

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T-Lymphocytes
Antigens
Genes
Phosphates
Artificial Cells
Superantigens
Jurkat Cells
Burkitt Lymphoma
Cell Extracts
Mycobacterium tuberculosis
Interleukin-2
Transfection
Complementary DNA
Cell Proliferation
Peptides

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Bukowski, J. F., Morita, C. T., Tanaka, Y., Bloom, B. R., Brenner, M. B., & Band, H. (1995). Vγ2Vδ2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer. Journal of Immunology, 154(3), 998-1006.

Vγ2Vδ2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer. / Bukowski, J. F.; Morita, C. T.; Tanaka, Y.; Bloom, B. R.; Brenner, M. B.; Band, Hamid.

In: Journal of Immunology, Vol. 154, No. 3, 01.01.1995, p. 998-1006.

Research output: Contribution to journalArticle

Bukowski, JF, Morita, CT, Tanaka, Y, Bloom, BR, Brenner, MB & Band, H 1995, 'Vγ2Vδ2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer', Journal of Immunology, vol. 154, no. 3, pp. 998-1006.
Bukowski, J. F. ; Morita, C. T. ; Tanaka, Y. ; Bloom, B. R. ; Brenner, M. B. ; Band, Hamid. / Vγ2Vδ2 TCR-dependent recognition of non-peptide antigens and Daudi cells analyzed by TCR gene transfer. In: Journal of Immunology. 1995 ; Vol. 154, No. 3. pp. 998-1006.
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