Utilization of biotin in proliferating human lymphocytes

Janos Zempleni, Donald M. Mock

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Lymphocytes are part of the immune system and respond to antigenic stimulation with proliferation. We sought to determine whether mitogen- stimulated, proliferating lymphocytes increase the cellular uptake of biotin and, if so, to identify mechanisms that mediate the increase. Lymphocytes were isolated from human peripheral blood; proliferation of lymphocytes was induced by incubation with pokeweed lectin, concanavalin A or phytohemagglutinin. Biotin uptake was quantitated by determination of [3H] uptake into the lymphocytes during incubation with [3H]biotin after establishing that [3H]biotin is not metabolized within the lymphocytes during the incubation period (<5%). Biotin uptake into proliferating lymphocytes increased to 278-722% of the control values for nonproliferating lymphocytes. Kinetic analysis of biotin transport provided evidence that the increase is mediated by an increased number of transporters on the cell surface rather than by an increase in transporter affinity. Cycloheximide, an inhibitor of protein synthesis, completely suppressed the mitogen-stimulated increase in biotin transport. This observation is consistent with the hypothesis that proliferating lymphocytes increase biotin uptake by increasing the synthesis of new transporters. Biotin affinity and structural specificity were similar in proliferating and nonproliferating lymphocytes, suggesting that mitogens induced an increase in the number of the same transporter molecule that mediates transport in unstimulated lymphocytes. Mitogen-stimulated lymphocytes exhibited 2.5 times greater activities of biotin-dependent β-methylcrotonyl-CoA carboxylase compared with time 0 (at 72 h after addition of mitogen). This observation is consistent with the hypothesis that proliferating lymphocytes increase biotin uptake at least in part to provide adequate coenzyme for biotin-dependent carboxylases.

Original languageEnglish (US)
Pages (from-to)335S-337S
JournalJournal of Nutrition
Volume130
Issue number2 SUPPL.
StatePublished - Feb 10 2000

Fingerprint

biotin
Biotin
lymphocytes
Lymphocytes
Mitogens
uptake mechanisms
transporters
biotin carboxylase
protein synthesis inhibitors
Phytolacca americana
Pokeweed Mitogens
coenzymes
phytohemagglutinin
Protein Synthesis Inhibitors
cycloheximide
lymphocyte proliferation
concanavalin A
Coenzymes
Phytohemagglutinins
Cycloheximide

Keywords

  • Biotin
  • Carboxylases
  • Humans
  • Lymphocytes
  • Transport

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Utilization of biotin in proliferating human lymphocytes. / Zempleni, Janos; Mock, Donald M.

In: Journal of Nutrition, Vol. 130, No. 2 SUPPL., 10.02.2000, p. 335S-337S.

Research output: Contribution to journalArticle

Zempleni, J & Mock, DM 2000, 'Utilization of biotin in proliferating human lymphocytes', Journal of Nutrition, vol. 130, no. 2 SUPPL., pp. 335S-337S.
Zempleni, Janos ; Mock, Donald M. / Utilization of biotin in proliferating human lymphocytes. In: Journal of Nutrition. 2000 ; Vol. 130, No. 2 SUPPL. pp. 335S-337S.
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