Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera

Simuliidae)

K. P. Pruess, B. J. Adams, T. J. Parsons, X. Zhu, Thomas O Powers

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The complete mitochondrial cytochrome oxidase II gene was sequenced from 17 black flies, representing 13 putative species, and used to infer phylogenetic relationships. A midge (Paratanytarsus sp.) and three mosquitoes (Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus) were used as outgroup taxa. All outgroup taxa were highly divergent from black flies. Phylogenetic trees based on weighted parsimony (a priori and a posteriori), maximum likelihood, and neighbor-joining (log-determinant distances) differed topologically, with deeper nodes being the least well-supported. All analyses supported current classification into species groups but relationships among those groups were poorly resolved. The majority of phylogenetic signal came from closely related sister taxa. The CO-II gene may be useful for exploring relationships at or below the subgeneric level, but is of questionable value at higher taxonomic levels. The weighting method employed gave phylogenetic results similar to those reported by other authors for other insect CO-II data sets. A best estimate of phylogenetic relationships based on the CO-II gene is presented and discussed in relation to current black fly classification. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)286-295
Number of pages10
JournalMolecular Phylogenetics and Evolution
Volume16
Issue number2
DOIs
StatePublished - Jan 1 2000

Fingerprint

Simuliidae
Electron Transport Complex IV
Carbon Monoxide
Diptera
cytochrome-c oxidase
cytochrome
phylogenetics
gene
phylogeny
Genes
Culex
Anopheles
genes
Aedes
Culicidae
Insects
Anopheles quadrimaculatus
Culex quinquefasciatus
midges
Aedes aegypti

Keywords

  • Black fly
  • Character weighting
  • Cytochrome oxidase subunit II
  • Mitochondrial DNA
  • Phylogeny
  • Simuliidae

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera : Simuliidae). / Pruess, K. P.; Adams, B. J.; Parsons, T. J.; Zhu, X.; Powers, Thomas O.

In: Molecular Phylogenetics and Evolution, Vol. 16, No. 2, 01.01.2000, p. 286-295.

Research output: Contribution to journalArticle

@article{321cf17d1c834ef289fcd08ea007758a,
title = "Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera: Simuliidae)",
abstract = "The complete mitochondrial cytochrome oxidase II gene was sequenced from 17 black flies, representing 13 putative species, and used to infer phylogenetic relationships. A midge (Paratanytarsus sp.) and three mosquitoes (Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus) were used as outgroup taxa. All outgroup taxa were highly divergent from black flies. Phylogenetic trees based on weighted parsimony (a priori and a posteriori), maximum likelihood, and neighbor-joining (log-determinant distances) differed topologically, with deeper nodes being the least well-supported. All analyses supported current classification into species groups but relationships among those groups were poorly resolved. The majority of phylogenetic signal came from closely related sister taxa. The CO-II gene may be useful for exploring relationships at or below the subgeneric level, but is of questionable value at higher taxonomic levels. The weighting method employed gave phylogenetic results similar to those reported by other authors for other insect CO-II data sets. A best estimate of phylogenetic relationships based on the CO-II gene is presented and discussed in relation to current black fly classification. (C) 2000 Academic Press.",
keywords = "Black fly, Character weighting, Cytochrome oxidase subunit II, Mitochondrial DNA, Phylogeny, Simuliidae",
author = "Pruess, {K. P.} and Adams, {B. J.} and Parsons, {T. J.} and X. Zhu and Powers, {Thomas O}",
year = "2000",
month = "1",
day = "1",
doi = "10.1006/mpev.2000.0807",
language = "English (US)",
volume = "16",
pages = "286--295",
journal = "Molecular Phylogenetics and Evolution",
issn = "1055-7903",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera

T2 - Simuliidae)

AU - Pruess, K. P.

AU - Adams, B. J.

AU - Parsons, T. J.

AU - Zhu, X.

AU - Powers, Thomas O

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The complete mitochondrial cytochrome oxidase II gene was sequenced from 17 black flies, representing 13 putative species, and used to infer phylogenetic relationships. A midge (Paratanytarsus sp.) and three mosquitoes (Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus) were used as outgroup taxa. All outgroup taxa were highly divergent from black flies. Phylogenetic trees based on weighted parsimony (a priori and a posteriori), maximum likelihood, and neighbor-joining (log-determinant distances) differed topologically, with deeper nodes being the least well-supported. All analyses supported current classification into species groups but relationships among those groups were poorly resolved. The majority of phylogenetic signal came from closely related sister taxa. The CO-II gene may be useful for exploring relationships at or below the subgeneric level, but is of questionable value at higher taxonomic levels. The weighting method employed gave phylogenetic results similar to those reported by other authors for other insect CO-II data sets. A best estimate of phylogenetic relationships based on the CO-II gene is presented and discussed in relation to current black fly classification. (C) 2000 Academic Press.

AB - The complete mitochondrial cytochrome oxidase II gene was sequenced from 17 black flies, representing 13 putative species, and used to infer phylogenetic relationships. A midge (Paratanytarsus sp.) and three mosquitoes (Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus) were used as outgroup taxa. All outgroup taxa were highly divergent from black flies. Phylogenetic trees based on weighted parsimony (a priori and a posteriori), maximum likelihood, and neighbor-joining (log-determinant distances) differed topologically, with deeper nodes being the least well-supported. All analyses supported current classification into species groups but relationships among those groups were poorly resolved. The majority of phylogenetic signal came from closely related sister taxa. The CO-II gene may be useful for exploring relationships at or below the subgeneric level, but is of questionable value at higher taxonomic levels. The weighting method employed gave phylogenetic results similar to those reported by other authors for other insect CO-II data sets. A best estimate of phylogenetic relationships based on the CO-II gene is presented and discussed in relation to current black fly classification. (C) 2000 Academic Press.

KW - Black fly

KW - Character weighting

KW - Cytochrome oxidase subunit II

KW - Mitochondrial DNA

KW - Phylogeny

KW - Simuliidae

UR - http://www.scopus.com/inward/record.url?scp=0033850416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033850416&partnerID=8YFLogxK

U2 - 10.1006/mpev.2000.0807

DO - 10.1006/mpev.2000.0807

M3 - Article

VL - 16

SP - 286

EP - 295

JO - Molecular Phylogenetics and Evolution

JF - Molecular Phylogenetics and Evolution

SN - 1055-7903

IS - 2

ER -