Utility of 2-D and 3-D virtual microscopy in cervical cytology education and testing

Fred R. Dee, Amber D Donnelly, Stanley J Radio, Timothy Leaven, M. Sue Zaleski, Clarence Kreiter

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: To evaluate the effectiveness of 3-D vs. 2-D virtual microscopy as adjuncts to education and assessment in cervical cytology. Study Design: Five cervical cytology slides were acquired in 2-D; then the identical area of the slide was acquired in 3-D, resulting in 2 sets of virtual slides for comparison with the original glass slide. Seventy-nine paid volunteer cytologists and cytotechnology students participated. Approximately half were sent the 2-D set of slides via the Web, and the others a 3-D set of slides on a DVD. Evaluators examined the virtual slides and committed to an interpretation. After receipt of the original glass slides, a second interpretation was made, if different from the virtual slide interpretation. Results: Diagnostic accuracy using virtual cytology slides was similar to that for glass slides (94% vs. 96%). There was no difference in diagnostic accuracy between 2-D and 3-D slides (p = 0.28); however, the ability to focus 3-D slides in the z-axis was strongly endorsed by the participants because of the uncertainty and frustration of having some cells out of focus on 2-D virtual slides. Conclusion: There was consensus that virtual cervical cytology slides would be a useful augmentation to education and testing.

Original languageEnglish (US)
Pages (from-to)523-529
Number of pages7
JournalActa Cytologica
Volume51
Issue number4
DOIs
StatePublished - Jan 1 2007

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Cell Biology
Microscopy
Glass
Education
Aptitude
Frustration
Uncertainty
Volunteers
Consensus
Students

Keywords

  • Cytology
  • Lesion, squamous intraepithelial
  • Virtual microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Utility of 2-D and 3-D virtual microscopy in cervical cytology education and testing. / Dee, Fred R.; Donnelly, Amber D; Radio, Stanley J; Leaven, Timothy; Zaleski, M. Sue; Kreiter, Clarence.

In: Acta Cytologica, Vol. 51, No. 4, 01.01.2007, p. 523-529.

Research output: Contribution to journalArticle

Dee, Fred R. ; Donnelly, Amber D ; Radio, Stanley J ; Leaven, Timothy ; Zaleski, M. Sue ; Kreiter, Clarence. / Utility of 2-D and 3-D virtual microscopy in cervical cytology education and testing. In: Acta Cytologica. 2007 ; Vol. 51, No. 4. pp. 523-529.
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