Using NMR metabolomics to investigate tricarboxylic acid cycle-dependent signal transduction in Staphylococcus epidermidis

Marat R. Sadykov, Bo Zhang, Steven Halouska, Jennifer L. Nelson, Lauren W. Kreimer, Yefei Zhu, Robert Powers, Greg A Somerville

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Staphylococcus epidermidis is a skin-resident bacterium and a major cause of biomaterial-associated infections. The transition from residing on the skin to residing on an implanted biomaterial is accompanied by regulatory changes that facilitate bacterial survival in the new environment. These regulatory changes are dependent upon the ability of bacteria to "sense" environmental changes. In S. epidermidis, disparate environmental signals can affect synthesis of the biofilm matrix polysaccharide intercellular adhesin (PIA). Previously, we demonstrated that PIA biosynthesis is regulated by tricarboxylic acid (TCA) cycle activity. The observations that very different environmental signals result in a common phenotype (i.e. increased PIA synthesis) and that TCA cycle activity regulates PIA biosynthesis led us to hypothesize that S. epidermidis is "sensing" disparate environmental signals through the modulation of TCA cycle activity. In this study, we used NMR metabolomics to demonstrate that divergent environmental signals are transduced into common metabolomic changes that are "sensed" by metabolite-responsive regulators, such as CcpA, to affect PIA biosynthesis. These data clarify one mechanism by which very different environmental signals cause common phenotypic changes. In addition, due to the frequency of the TCA cycle in diverse genera of bacteria and the intrinsic properties of TCA cycle enzymes, it is likely the TCA cycle acts as a signal transduction pathway in many bacteria.

Original languageEnglish (US)
Pages (from-to)36616-36624
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number47
DOIs
StatePublished - Nov 19 2010

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Signal transduction
Metabolomics
Citric Acid Cycle
Staphylococcus epidermidis
Signal Transduction
Nuclear magnetic resonance
Biosynthesis
Bacteria
Biocompatible Materials
Skin
Biofilms
Metabolites
Modulation
polysaccharide intercellular adhesin
Phenotype
Enzymes
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Using NMR metabolomics to investigate tricarboxylic acid cycle-dependent signal transduction in Staphylococcus epidermidis. / Sadykov, Marat R.; Zhang, Bo; Halouska, Steven; Nelson, Jennifer L.; Kreimer, Lauren W.; Zhu, Yefei; Powers, Robert; Somerville, Greg A.

In: Journal of Biological Chemistry, Vol. 285, No. 47, 19.11.2010, p. 36616-36624.

Research output: Contribution to journalArticle

Sadykov, Marat R. ; Zhang, Bo ; Halouska, Steven ; Nelson, Jennifer L. ; Kreimer, Lauren W. ; Zhu, Yefei ; Powers, Robert ; Somerville, Greg A. / Using NMR metabolomics to investigate tricarboxylic acid cycle-dependent signal transduction in Staphylococcus epidermidis. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 47. pp. 36616-36624.
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