Using DArT markers to monitor genetic diversity throughout selection: A case study in Nebraska's winter wheat breeding nurseries

Ibrahim El-Basyoni, P. Stephen Baenziger, Ismail Dweikat, Dong Wang, Kent Eskridge, Mohamed Saadalla

Research output: Contribution to journalArticle

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Abstract

Assessing the genetic relationship among wheat (Triticum aestivum L.) lines in the intermediate generations of a breeding program has important consequences on conserving genetic variability for selection in later generations. The objectives of this study were to (i) determine the breadth of the genetic base of two F3:6 nurseries and (ii) to monitor the effect of selection on the genetic diversity. Two independent F3:6 nurseries (2010 and 2011 seasons) were used; the first nursery contained 276 lines and two local check cultivars genotyped using 1925 polymorphic diversity array technology (DArT) markers while the second nursery contained 278 lines plus the same local check cultivars genotyped using 2236 polymorphic DArT markers. The F3:6 nurseries, 2010 and 2011, were each grouped into three main clusters. Overall, the results suggested that, in both years, the difference among clusters was significant, and the genetic diversity in the F3:6 nurseries exceeded that between the two check cultivars. The results for the two nurseries showed that each cluster in the F3:6 nurseries was represented in the F3:7 and F3:8 with at least one line, except for one cluster in the 2011 nursery which was not represented in F3:8. We concluded that much of the genetic diversity was maintained while advancing lines from F3:6 to F3:7; however, as the selection intensity increases, for example from F3:7 to F3:8, the effect of selection and the importance of monitoring genetic diversity using DNA molecular markers increases.

Original languageEnglish (US)
Pages (from-to)2363-2373
Number of pages11
JournalCrop Science
Volume53
Issue number6
DOIs
StatePublished - Nov 1 2013

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winter wheat
case studies
genetic variation
breeding
cultivars
selection intensity
monitoring
genetic background
genetic relationships
Triticum aestivum
wheat
genetic markers
DNA

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Using DArT markers to monitor genetic diversity throughout selection : A case study in Nebraska's winter wheat breeding nurseries. / El-Basyoni, Ibrahim; Baenziger, P. Stephen; Dweikat, Ismail; Wang, Dong; Eskridge, Kent; Saadalla, Mohamed.

In: Crop Science, Vol. 53, No. 6, 01.11.2013, p. 2363-2373.

Research output: Contribution to journalArticle

El-Basyoni, Ibrahim ; Baenziger, P. Stephen ; Dweikat, Ismail ; Wang, Dong ; Eskridge, Kent ; Saadalla, Mohamed. / Using DArT markers to monitor genetic diversity throughout selection : A case study in Nebraska's winter wheat breeding nurseries. In: Crop Science. 2013 ; Vol. 53, No. 6. pp. 2363-2373.
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