Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria

Role of D-alanine racemase

Steven Halouska, Ofelia Chacon, Robert J. Fenton, Denise K. Zinniel, Raul G Barletta, Robert Powers

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.

Original languageEnglish (US)
Pages (from-to)4608-4614
Number of pages7
JournalJournal of Proteome Research
Volume6
Issue number12
DOIs
StatePublished - Dec 1 2007

Fingerprint

Alanine Racemase
Cycloserine
Metabolomics
Mycobacterium
Nuclear magnetic resonance
Mycobacterium smegmatis
Multidrug-Resistant Tuberculosis
Biosynthesis
Principal Component Analysis
Principal component analysis
Cell Wall
Cells
Proteins

Keywords

  • D-alanine racemase
  • D-cycloserine
  • Metabolomics
  • Mycobacterium smegmatis
  • NMR
  • Principal component analysis
  • Tuberculosis

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria : Role of D-alanine racemase. / Halouska, Steven; Chacon, Ofelia; Fenton, Robert J.; Zinniel, Denise K.; Barletta, Raul G; Powers, Robert.

In: Journal of Proteome Research, Vol. 6, No. 12, 01.12.2007, p. 4608-4614.

Research output: Contribution to journalArticle

Halouska, Steven ; Chacon, Ofelia ; Fenton, Robert J. ; Zinniel, Denise K. ; Barletta, Raul G ; Powers, Robert. / Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria : Role of D-alanine racemase. In: Journal of Proteome Research. 2007 ; Vol. 6, No. 12. pp. 4608-4614.
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