Use of monoclonal antibodies, morphology, and cytochemistry to probe the cellular heterogeneity of acute leukemia and lymphoma

Tucker W. LeBien, Robert W. McKenna, Candice S. Abramson, Kazimiera J. Gail-Peczalska, Mark E. Nesbit, Peter Felix Coccia, Clara D. Bloomfield, Richard D. Brunning, John H. Kersey

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Abstract

A combined immunological, morphological, and cytochemical approach to the study of malignant cells in patients with acute leukemia and lymphoma is presented. Newly produced monoclonal antibodies that bind to antigens of human mononuclear cells (TA-1), or B-lymphocytes (BA-1) were used to study malignant cells from patients with acute lymphoblastic leukemia (ALL), acute myelocytic leukemia, acute myelomonocytic leukemia, and chronic lymphocytic leukemia. Results in lymphoid leukemia-lymphoma patients were compared with other immunological markers and indicate that the major groups of ALL and childhood non-Hodgkin’s lymphoma are T-ALL, pre-T-ALL, pre-B-ALL, B-ALL, and non-T, non-B-ALL. In addition, each major group had multiple phenotypes when analyzed with seven immunological markers including the erythrocyte rosette receptor, surface immunoglobulin, cytoplasmic immunoglobulin M, the early lymphocyte-acute lymphoblastic leukemia antigen, monoclonal antibody TA-1, monoclonal antibody BA-1, and a monoclonal antibody against HLA-DR. While immunological heterogeneity was demonstrable within each group, distinct biological behavior was observed, with T-ALL and B-ALL generally presenting as “lymphomas” and the others presenting as “leukemias.” Morphological analysis using the French-American-British classification provided independent information in the definition of groups with differing clinical behavior. Cytochemical analyses demonstrated focal paranuclear staining of leukemia cells with acid phosphatase in 73% of T-ALLs and 6% of non-T, non-B-ALLs. Downloaded from cancerres.aacrjournals.org on February 9, 2016.

Original languageEnglish (US)
Pages (from-to)4776-4780
Number of pages5
JournalCancer Research
Volume41
StatePublished - Nov 1 1981

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Histocytochemistry
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Lymphoma
Leukemia
Monoclonal Antibodies
Leukemia, Myelomonocytic, Acute
Lymphoid Leukemia
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Antigens
B-Cell Antigen Receptors
HLA-DR Antigens
B-Cell Chronic Lymphocytic Leukemia
Acid Phosphatase
Acute Myeloid Leukemia
Non-Hodgkin's Lymphoma
Immunoglobulin M
B-Lymphocytes
Erythrocytes
Lymphocytes
Staining and Labeling

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

LeBien, T. W., McKenna, R. W., Abramson, C. S., Gail-Peczalska, K. J., Nesbit, M. E., Coccia, P. F., ... Kersey, J. H. (1981). Use of monoclonal antibodies, morphology, and cytochemistry to probe the cellular heterogeneity of acute leukemia and lymphoma. Cancer Research, 41, 4776-4780.

Use of monoclonal antibodies, morphology, and cytochemistry to probe the cellular heterogeneity of acute leukemia and lymphoma. / LeBien, Tucker W.; McKenna, Robert W.; Abramson, Candice S.; Gail-Peczalska, Kazimiera J.; Nesbit, Mark E.; Coccia, Peter Felix; Bloomfield, Clara D.; Brunning, Richard D.; Kersey, John H.

In: Cancer Research, Vol. 41, 01.11.1981, p. 4776-4780.

Research output: Contribution to journalArticle

LeBien, TW, McKenna, RW, Abramson, CS, Gail-Peczalska, KJ, Nesbit, ME, Coccia, PF, Bloomfield, CD, Brunning, RD & Kersey, JH 1981, 'Use of monoclonal antibodies, morphology, and cytochemistry to probe the cellular heterogeneity of acute leukemia and lymphoma', Cancer Research, vol. 41, pp. 4776-4780.
LeBien TW, McKenna RW, Abramson CS, Gail-Peczalska KJ, Nesbit ME, Coccia PF et al. Use of monoclonal antibodies, morphology, and cytochemistry to probe the cellular heterogeneity of acute leukemia and lymphoma. Cancer Research. 1981 Nov 1;41:4776-4780.
LeBien, Tucker W. ; McKenna, Robert W. ; Abramson, Candice S. ; Gail-Peczalska, Kazimiera J. ; Nesbit, Mark E. ; Coccia, Peter Felix ; Bloomfield, Clara D. ; Brunning, Richard D. ; Kersey, John H. / Use of monoclonal antibodies, morphology, and cytochemistry to probe the cellular heterogeneity of acute leukemia and lymphoma. In: Cancer Research. 1981 ; Vol. 41. pp. 4776-4780.
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abstract = "A combined immunological, morphological, and cytochemical approach to the study of malignant cells in patients with acute leukemia and lymphoma is presented. Newly produced monoclonal antibodies that bind to antigens of human mononuclear cells (TA-1), or B-lymphocytes (BA-1) were used to study malignant cells from patients with acute lymphoblastic leukemia (ALL), acute myelocytic leukemia, acute myelomonocytic leukemia, and chronic lymphocytic leukemia. Results in lymphoid leukemia-lymphoma patients were compared with other immunological markers and indicate that the major groups of ALL and childhood non-Hodgkin’s lymphoma are T-ALL, pre-T-ALL, pre-B-ALL, B-ALL, and non-T, non-B-ALL. In addition, each major group had multiple phenotypes when analyzed with seven immunological markers including the erythrocyte rosette receptor, surface immunoglobulin, cytoplasmic immunoglobulin M, the early lymphocyte-acute lymphoblastic leukemia antigen, monoclonal antibody TA-1, monoclonal antibody BA-1, and a monoclonal antibody against HLA-DR. While immunological heterogeneity was demonstrable within each group, distinct biological behavior was observed, with T-ALL and B-ALL generally presenting as “lymphomas” and the others presenting as “leukemias.” Morphological analysis using the French-American-British classification provided independent information in the definition of groups with differing clinical behavior. Cytochemical analyses demonstrated focal paranuclear staining of leukemia cells with acid phosphatase in 73{\%} of T-ALLs and 6{\%} of non-T, non-B-ALLs. Downloaded from cancerres.aacrjournals.org on February 9, 2016.",
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N2 - A combined immunological, morphological, and cytochemical approach to the study of malignant cells in patients with acute leukemia and lymphoma is presented. Newly produced monoclonal antibodies that bind to antigens of human mononuclear cells (TA-1), or B-lymphocytes (BA-1) were used to study malignant cells from patients with acute lymphoblastic leukemia (ALL), acute myelocytic leukemia, acute myelomonocytic leukemia, and chronic lymphocytic leukemia. Results in lymphoid leukemia-lymphoma patients were compared with other immunological markers and indicate that the major groups of ALL and childhood non-Hodgkin’s lymphoma are T-ALL, pre-T-ALL, pre-B-ALL, B-ALL, and non-T, non-B-ALL. In addition, each major group had multiple phenotypes when analyzed with seven immunological markers including the erythrocyte rosette receptor, surface immunoglobulin, cytoplasmic immunoglobulin M, the early lymphocyte-acute lymphoblastic leukemia antigen, monoclonal antibody TA-1, monoclonal antibody BA-1, and a monoclonal antibody against HLA-DR. While immunological heterogeneity was demonstrable within each group, distinct biological behavior was observed, with T-ALL and B-ALL generally presenting as “lymphomas” and the others presenting as “leukemias.” Morphological analysis using the French-American-British classification provided independent information in the definition of groups with differing clinical behavior. Cytochemical analyses demonstrated focal paranuclear staining of leukemia cells with acid phosphatase in 73% of T-ALLs and 6% of non-T, non-B-ALLs. Downloaded from cancerres.aacrjournals.org on February 9, 2016.

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