Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter discusses the use of hepatocytes to study ethanol metabolism and hepatotoxicity. Alcoholic liver disease is a complex, multifactoral disease that normally requires many years of alcohol abuse to develop. The fact that alcoholic liver disease requires this long period to develop is most likely to be a consequence of the compensatory or adaptive responses of the liver, and the tremendous capacity of the liver to replace damaged cells or regenerate after toxic injury. These facts have made studies using laboratory animals difficult. In fact, there is no convenient animal model that exhibits the full spectrum of pathology associated with alcoholic liver disease. Hepatocytes isolated from the liver, rapidly dedifferentiate and lose the ability to metabolize ethanol. Because of this, until recently, it has not been possible to investigate the effects of prolonged ethanol metabolism in vitro. Using genetic engineering techniques, a number of recombinant, hepatic cell lines have been created that express the enzymes responsible for the metabolism of ethanol. Although the use of these cell lines has limitations, but many mechanistic studies have been completed that previously were difficult if not impossible to perform. Ethanol metabolism results in many physiologic changes in hepatocytes that occur simultaneously.

Original languageEnglish (US)
Title of host publicationComprehensive Handbook of Alcohol Related Pathology
PublisherElsevier Inc.
Pages1559-1572
Number of pages14
Volume3-3
ISBN (Electronic)9780080502311
ISBN (Print)9780125643702
DOIs
StatePublished - Jan 1 2005

Fingerprint

Liver
Ethanol
Alcoholic Liver Diseases
Hepatocytes
Metabolism
Animals
Genetic Techniques
Cell Line
Aptitude
Genetic Engineering
Poisons
Laboratory Animals
Genetic engineering
Alcoholism
Pathology
Animal Models
Cells
Alcohols
Wounds and Injuries
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Clemens, D. L. (2005). Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity. In Comprehensive Handbook of Alcohol Related Pathology (Vol. 3-3, pp. 1559-1572). Elsevier Inc.. https://doi.org/10.1016/B978-012564370-2/50117-3

Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity. / Clemens, Dahn L.

Comprehensive Handbook of Alcohol Related Pathology. Vol. 3-3 Elsevier Inc., 2005. p. 1559-1572.

Research output: Chapter in Book/Report/Conference proceedingChapter

Clemens, DL 2005, Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity. in Comprehensive Handbook of Alcohol Related Pathology. vol. 3-3, Elsevier Inc., pp. 1559-1572. https://doi.org/10.1016/B978-012564370-2/50117-3
Clemens DL. Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity. In Comprehensive Handbook of Alcohol Related Pathology. Vol. 3-3. Elsevier Inc. 2005. p. 1559-1572 https://doi.org/10.1016/B978-012564370-2/50117-3
Clemens, Dahn L. / Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity. Comprehensive Handbook of Alcohol Related Pathology. Vol. 3-3 Elsevier Inc., 2005. pp. 1559-1572
@inbook{b1ba972ed658412b82d4719cca14fdda,
title = "Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity",
abstract = "This chapter discusses the use of hepatocytes to study ethanol metabolism and hepatotoxicity. Alcoholic liver disease is a complex, multifactoral disease that normally requires many years of alcohol abuse to develop. The fact that alcoholic liver disease requires this long period to develop is most likely to be a consequence of the compensatory or adaptive responses of the liver, and the tremendous capacity of the liver to replace damaged cells or regenerate after toxic injury. These facts have made studies using laboratory animals difficult. In fact, there is no convenient animal model that exhibits the full spectrum of pathology associated with alcoholic liver disease. Hepatocytes isolated from the liver, rapidly dedifferentiate and lose the ability to metabolize ethanol. Because of this, until recently, it has not been possible to investigate the effects of prolonged ethanol metabolism in vitro. Using genetic engineering techniques, a number of recombinant, hepatic cell lines have been created that express the enzymes responsible for the metabolism of ethanol. Although the use of these cell lines has limitations, but many mechanistic studies have been completed that previously were difficult if not impossible to perform. Ethanol metabolism results in many physiologic changes in hepatocytes that occur simultaneously.",
author = "Clemens, {Dahn L}",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/B978-012564370-2/50117-3",
language = "English (US)",
isbn = "9780125643702",
volume = "3-3",
pages = "1559--1572",
booktitle = "Comprehensive Handbook of Alcohol Related Pathology",
publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Use of Isolated Cells in the Study of Ethanol-Induced Hepatotoxicity

AU - Clemens, Dahn L

PY - 2005/1/1

Y1 - 2005/1/1

N2 - This chapter discusses the use of hepatocytes to study ethanol metabolism and hepatotoxicity. Alcoholic liver disease is a complex, multifactoral disease that normally requires many years of alcohol abuse to develop. The fact that alcoholic liver disease requires this long period to develop is most likely to be a consequence of the compensatory or adaptive responses of the liver, and the tremendous capacity of the liver to replace damaged cells or regenerate after toxic injury. These facts have made studies using laboratory animals difficult. In fact, there is no convenient animal model that exhibits the full spectrum of pathology associated with alcoholic liver disease. Hepatocytes isolated from the liver, rapidly dedifferentiate and lose the ability to metabolize ethanol. Because of this, until recently, it has not been possible to investigate the effects of prolonged ethanol metabolism in vitro. Using genetic engineering techniques, a number of recombinant, hepatic cell lines have been created that express the enzymes responsible for the metabolism of ethanol. Although the use of these cell lines has limitations, but many mechanistic studies have been completed that previously were difficult if not impossible to perform. Ethanol metabolism results in many physiologic changes in hepatocytes that occur simultaneously.

AB - This chapter discusses the use of hepatocytes to study ethanol metabolism and hepatotoxicity. Alcoholic liver disease is a complex, multifactoral disease that normally requires many years of alcohol abuse to develop. The fact that alcoholic liver disease requires this long period to develop is most likely to be a consequence of the compensatory or adaptive responses of the liver, and the tremendous capacity of the liver to replace damaged cells or regenerate after toxic injury. These facts have made studies using laboratory animals difficult. In fact, there is no convenient animal model that exhibits the full spectrum of pathology associated with alcoholic liver disease. Hepatocytes isolated from the liver, rapidly dedifferentiate and lose the ability to metabolize ethanol. Because of this, until recently, it has not been possible to investigate the effects of prolonged ethanol metabolism in vitro. Using genetic engineering techniques, a number of recombinant, hepatic cell lines have been created that express the enzymes responsible for the metabolism of ethanol. Although the use of these cell lines has limitations, but many mechanistic studies have been completed that previously were difficult if not impossible to perform. Ethanol metabolism results in many physiologic changes in hepatocytes that occur simultaneously.

UR - http://www.scopus.com/inward/record.url?scp=84943269701&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943269701&partnerID=8YFLogxK

U2 - 10.1016/B978-012564370-2/50117-3

DO - 10.1016/B978-012564370-2/50117-3

M3 - Chapter

SN - 9780125643702

VL - 3-3

SP - 1559

EP - 1572

BT - Comprehensive Handbook of Alcohol Related Pathology

PB - Elsevier Inc.

ER -