Abstract
Effective therapeutic application of gene drugs requires noninvasive and cost-effective methods for gene delivery, which should be safe for repetitive use and provide reproducible therapeutic effect. Systematic research efforts in the area of polyelectrolyte complexes of DNA with polycations (polyplexes) resulted in an increased understanding of the principles governing the biological activity of polyplexes and now permit rational design of increasingly more efficient vectors. Despite this tremendous progress, significant constrains limiting the efficiency of the gene delivery process mediated by polyplexes still remain, particularly when they are administered systemically.1 The growing emphasis on the systemic delivery of genes via intravenous injection reflects the need to gain access to disseminated and widespread disease targets, such as cancer cells, and to expand the available therapeutic modalities. To construct a delivery system suitable for systemic administration, a thorough understanding of the in vivo pharmacokinetic and disposition characteristics of the vector is important.2 The distribution and elimination patterns of systemically administered polyplexes largely follow general behavior observed for other macromolecules and nanoparticles, and depend mainly on the physicochemical properties of the carrier, such as size and molecular weight, electric charge, and hydrophilic/hydrophobic balance.3,4 Based on the accurate understanding of the relationship between the physicochemical properties of macromolecular carriers and their pharmacokinetics, it is often possible to effectively control their disposition properties.
| Original language | English (US) |
|---|---|
| Title of host publication | Polymeric Gene Delivery |
| Subtitle of host publication | Principles and Applications |
| Publisher | CRC Press |
| Pages | 345-354 |
| Number of pages | 10 |
| ISBN (Electronic) | 9780203500477 |
| ISBN (Print) | 9780849319341 |
| DOIs | |
| State | Published - Jan 1 2004 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Use of HPMA copolymers in gene delivery. / Oupicky, David.
Polymeric Gene Delivery: Principles and Applications. CRC Press, 2004. p. 345-354.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Use of HPMA copolymers in gene delivery
AU - Oupicky, David
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Effective therapeutic application of gene drugs requires noninvasive and cost-effective methods for gene delivery, which should be safe for repetitive use and provide reproducible therapeutic effect. Systematic research efforts in the area of polyelectrolyte complexes of DNA with polycations (polyplexes) resulted in an increased understanding of the principles governing the biological activity of polyplexes and now permit rational design of increasingly more efficient vectors. Despite this tremendous progress, significant constrains limiting the efficiency of the gene delivery process mediated by polyplexes still remain, particularly when they are administered systemically.1 The growing emphasis on the systemic delivery of genes via intravenous injection reflects the need to gain access to disseminated and widespread disease targets, such as cancer cells, and to expand the available therapeutic modalities. To construct a delivery system suitable for systemic administration, a thorough understanding of the in vivo pharmacokinetic and disposition characteristics of the vector is important.2 The distribution and elimination patterns of systemically administered polyplexes largely follow general behavior observed for other macromolecules and nanoparticles, and depend mainly on the physicochemical properties of the carrier, such as size and molecular weight, electric charge, and hydrophilic/hydrophobic balance.3,4 Based on the accurate understanding of the relationship between the physicochemical properties of macromolecular carriers and their pharmacokinetics, it is often possible to effectively control their disposition properties.
AB - Effective therapeutic application of gene drugs requires noninvasive and cost-effective methods for gene delivery, which should be safe for repetitive use and provide reproducible therapeutic effect. Systematic research efforts in the area of polyelectrolyte complexes of DNA with polycations (polyplexes) resulted in an increased understanding of the principles governing the biological activity of polyplexes and now permit rational design of increasingly more efficient vectors. Despite this tremendous progress, significant constrains limiting the efficiency of the gene delivery process mediated by polyplexes still remain, particularly when they are administered systemically.1 The growing emphasis on the systemic delivery of genes via intravenous injection reflects the need to gain access to disseminated and widespread disease targets, such as cancer cells, and to expand the available therapeutic modalities. To construct a delivery system suitable for systemic administration, a thorough understanding of the in vivo pharmacokinetic and disposition characteristics of the vector is important.2 The distribution and elimination patterns of systemically administered polyplexes largely follow general behavior observed for other macromolecules and nanoparticles, and depend mainly on the physicochemical properties of the carrier, such as size and molecular weight, electric charge, and hydrophilic/hydrophobic balance.3,4 Based on the accurate understanding of the relationship between the physicochemical properties of macromolecular carriers and their pharmacokinetics, it is often possible to effectively control their disposition properties.
UR - http://www.scopus.com/inward/record.url?scp=85055549406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055549406&partnerID=8YFLogxK
U2 - 10.1201/9780203500477
DO - 10.1201/9780203500477
M3 - Chapter
AN - SCOPUS:85055549406
SN - 9780849319341
SP - 345
EP - 354
BT - Polymeric Gene Delivery
PB - CRC Press
ER -