Use of gc × gc/tof-ms and lc/tof-ms for metabolomic analysis of hyalella azteca chronically exposed to atrazine and its primary metabolite, desethylatrazine

Kimberly J. Ralston-Hooper, Jiri Adamec, Amber Jannash, Robert Mollenhauer, Hugo Ochoa-Acuña, Maria S. Sepúlveda

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Atrazine is one of the most commonly detected contaminants in the U.S. Little information is available on one of atrazine's metabolites, desethylatrazine (DEA). Two-dimensional gas chromatography and liquid chromatography coupled with time of flight- mass spectrometry were used to examine metabolite profiles of Hyalella azteca chronically exposed to 30μg/L atrazine and DEA. The majority of identified metabolites were by-products of β-oxidation of fatty acids suggesting possible disruption in energy metabolism. Eicosanoids increased in exposed females suggesting possible perturbations in neuropeptide hormonal systems. Overall, this research demonstrates the feasibility of utilizing metabolomic profiling of invertebrate species exposed to environmental contaminants as a way to determine mechanisms of toxicity.

Original languageEnglish (US)
Pages (from-to)399-410
Number of pages12
JournalJournal of Applied Toxicology
Volume31
Issue number5
DOIs
StatePublished - Jul 1 2011

Fingerprint

Atrazine
Metabolomics
Metabolites
Impurities
Eicosanoids
Liquid chromatography
Invertebrates
Neuropeptides
Liquid Chromatography
Gas chromatography
Gas Chromatography
Energy Metabolism
Mass spectrometry
Byproducts
Toxicity
Mass Spectrometry
Fatty Acids
Oxidation
Research
desethylatrazine

Keywords

  • Atrazine
  • Desethylatrazine
  • Endocrine disruption
  • GC × GC/TOF-MS
  • Hormesis
  • Hyalella azteca
  • Invertebrates
  • LC/TOF-MS
  • Metabolomics

ASJC Scopus subject areas

  • Toxicology

Cite this

Use of gc × gc/tof-ms and lc/tof-ms for metabolomic analysis of hyalella azteca chronically exposed to atrazine and its primary metabolite, desethylatrazine. / Ralston-Hooper, Kimberly J.; Adamec, Jiri; Jannash, Amber; Mollenhauer, Robert; Ochoa-Acuña, Hugo; Sepúlveda, Maria S.

In: Journal of Applied Toxicology, Vol. 31, No. 5, 01.07.2011, p. 399-410.

Research output: Contribution to journalArticle

Ralston-Hooper, Kimberly J. ; Adamec, Jiri ; Jannash, Amber ; Mollenhauer, Robert ; Ochoa-Acuña, Hugo ; Sepúlveda, Maria S. / Use of gc × gc/tof-ms and lc/tof-ms for metabolomic analysis of hyalella azteca chronically exposed to atrazine and its primary metabolite, desethylatrazine. In: Journal of Applied Toxicology. 2011 ; Vol. 31, No. 5. pp. 399-410.
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AU - Adamec, Jiri

AU - Jannash, Amber

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AU - Ochoa-Acuña, Hugo

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