Abstract

Hepatobiliary diseases result in the accumulation of bile acids (BAs) in the liver, systemic blood, and other tissues leading to an unfavorable prognosis. The BA profile was characterized by the calculation of indices that describe the composition, sulfation, and amidation of total and individual BAs. Comparison of the urinary BA profiles between healthy subjects and patients with hepatobiliary diseases demonstrated significantly higher absolute concentrations of individual and total BAs in patients. The percentage sulfation of some individual BAs were different between the two groups. The percentage amidation of overall and most individual BAs was higher in patients than controls. The percentage of primary BAs (CDCA and CA) was higher in patients, whereas the percentage of secondary BAs (DCA and LCA) was lower in patients. BA indices belonging to percentage amidation and percentage composition were better associated with the severity of the liver disease as determined by the model for end-stage liver disease (MELD) score and disease compensation status compared with the absolute concentrations of individual and total BAs. In addition, BA indices corresponding to percentage amidation and percentage composition of certain BAs demonstrated the highest area under the receiver operating characteristic (ROC) curve suggesting their utility as diagnostic biomarkers in clinic. Furthermore, significant increase in the risk of having liver diseases was associated with changes in BA indices.

Original languageEnglish (US)
Article numberkfu228
Pages (from-to)308-318
Number of pages11
JournalToxicological Sciences
Volume143
Issue number2
DOIs
StatePublished - Feb 1 2015

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Biomarkers
Bile Acids and Salts
Liver
Liver Diseases
Chemical analysis
End Stage Liver Disease
ROC Curve
Healthy Volunteers
Blood

Keywords

  • Bile acid amidation
  • Bile acid sulfation
  • Bile acids
  • Biomarker
  • Hepatobiliary diseases

ASJC Scopus subject areas

  • Toxicology

Cite this

Urinary bile acids as biomarkers for liver diseases II. signature profiles in patients. / Bathena, Sai Praneeth R; Thakare, Rhishikesh; Gautam, Nagsen; Mukherjee, Sandeep; Olivera-Martinez, Marco A; Meza, Jane L; Alnouti, Yazen.

In: Toxicological Sciences, Vol. 143, No. 2, kfu228, 01.02.2015, p. 308-318.

Research output: Contribution to journalArticle

Bathena, Sai Praneeth R ; Thakare, Rhishikesh ; Gautam, Nagsen ; Mukherjee, Sandeep ; Olivera-Martinez, Marco A ; Meza, Jane L ; Alnouti, Yazen. / Urinary bile acids as biomarkers for liver diseases II. signature profiles in patients. In: Toxicological Sciences. 2015 ; Vol. 143, No. 2. pp. 308-318.
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