Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas

Fadia Ibrahim, Linda A. Rymarquis, Eun Jeong Kim, James Becker, Eniko Balassa, Pamela J. Green, Heriberto D Cerutti

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Regulation of gene expression by small RNAs(′20-30 nucleotides in length) plays an essential role in developmental pathways and defense responses against genomic parasites in eukaryotes. Micro-RNAs (miRNAs) and small interfering RNAs (siRNAs) commonly direct the inactivation of cognate sequences through a variety of mechanisms, including RNA degradation, translation inhibition, and transcriptional repression. Recent studies have provided considerable insight into the biogenesis and the mode of action of miRNAs and siRNAs. However, relatively little is known about mechanisms of quality control and small RNA decay in RNA interference (RNAi) pathways. Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels. We found that MUT68 plays a role in the untemplated uridylation of the 3′ ends of small RNAs in vivo and stimulates their degradation by the RRP6 exosome subunit in vitro. Moreover, RRP6 depletion also leads to accumulation of small RNAs in vivo. We propose that MUT68 and RRP6 cooperate in the degradation of mature miRNAs and siRNAs, as a quality control mechanism to eliminate dysfunctional or damaged small RNA molecules.

Original languageEnglish (US)
Pages (from-to)3906-3911
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number8
DOIs
StatePublished - Feb 23 2010

Fingerprint

RNA Nucleotidyltransferases
Chlamydomonas
MicroRNAs
Small Interfering RNA
RNA
RNA Stability
Quality Control
Nucleotidyltransferases
Exosomes
Chlamydomonas reinhardtii
Gene Expression Regulation
RNA Interference
Eukaryota
Parasites
Nucleotides

Keywords

  • Exosome
  • RNA interference
  • miRNA quality control

ASJC Scopus subject areas

  • General

Cite this

Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas. / Ibrahim, Fadia; Rymarquis, Linda A.; Kim, Eun Jeong; Becker, James; Balassa, Eniko; Green, Pamela J.; Cerutti, Heriberto D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 8, 23.02.2010, p. 3906-3911.

Research output: Contribution to journalArticle

Ibrahim, Fadia ; Rymarquis, Linda A. ; Kim, Eun Jeong ; Becker, James ; Balassa, Eniko ; Green, Pamela J. ; Cerutti, Heriberto D. / Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 8. pp. 3906-3911.
@article{9bcee4bc86374bdb85dfef70670e88c5,
title = "Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas",
abstract = "Regulation of gene expression by small RNAs(′20-30 nucleotides in length) plays an essential role in developmental pathways and defense responses against genomic parasites in eukaryotes. Micro-RNAs (miRNAs) and small interfering RNAs (siRNAs) commonly direct the inactivation of cognate sequences through a variety of mechanisms, including RNA degradation, translation inhibition, and transcriptional repression. Recent studies have provided considerable insight into the biogenesis and the mode of action of miRNAs and siRNAs. However, relatively little is known about mechanisms of quality control and small RNA decay in RNA interference (RNAi) pathways. Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels. We found that MUT68 plays a role in the untemplated uridylation of the 3′ ends of small RNAs in vivo and stimulates their degradation by the RRP6 exosome subunit in vitro. Moreover, RRP6 depletion also leads to accumulation of small RNAs in vivo. We propose that MUT68 and RRP6 cooperate in the degradation of mature miRNAs and siRNAs, as a quality control mechanism to eliminate dysfunctional or damaged small RNA molecules.",
keywords = "Exosome, RNA interference, miRNA quality control",
author = "Fadia Ibrahim and Rymarquis, {Linda A.} and Kim, {Eun Jeong} and James Becker and Eniko Balassa and Green, {Pamela J.} and Cerutti, {Heriberto D}",
year = "2010",
month = "2",
day = "23",
doi = "10.1073/pnas.0912632107",
language = "English (US)",
volume = "107",
pages = "3906--3911",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "8",

}

TY - JOUR

T1 - Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas

AU - Ibrahim, Fadia

AU - Rymarquis, Linda A.

AU - Kim, Eun Jeong

AU - Becker, James

AU - Balassa, Eniko

AU - Green, Pamela J.

AU - Cerutti, Heriberto D

PY - 2010/2/23

Y1 - 2010/2/23

N2 - Regulation of gene expression by small RNAs(′20-30 nucleotides in length) plays an essential role in developmental pathways and defense responses against genomic parasites in eukaryotes. Micro-RNAs (miRNAs) and small interfering RNAs (siRNAs) commonly direct the inactivation of cognate sequences through a variety of mechanisms, including RNA degradation, translation inhibition, and transcriptional repression. Recent studies have provided considerable insight into the biogenesis and the mode of action of miRNAs and siRNAs. However, relatively little is known about mechanisms of quality control and small RNA decay in RNA interference (RNAi) pathways. Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels. We found that MUT68 plays a role in the untemplated uridylation of the 3′ ends of small RNAs in vivo and stimulates their degradation by the RRP6 exosome subunit in vitro. Moreover, RRP6 depletion also leads to accumulation of small RNAs in vivo. We propose that MUT68 and RRP6 cooperate in the degradation of mature miRNAs and siRNAs, as a quality control mechanism to eliminate dysfunctional or damaged small RNA molecules.

AB - Regulation of gene expression by small RNAs(′20-30 nucleotides in length) plays an essential role in developmental pathways and defense responses against genomic parasites in eukaryotes. Micro-RNAs (miRNAs) and small interfering RNAs (siRNAs) commonly direct the inactivation of cognate sequences through a variety of mechanisms, including RNA degradation, translation inhibition, and transcriptional repression. Recent studies have provided considerable insight into the biogenesis and the mode of action of miRNAs and siRNAs. However, relatively little is known about mechanisms of quality control and small RNA decay in RNA interference (RNAi) pathways. Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels. We found that MUT68 plays a role in the untemplated uridylation of the 3′ ends of small RNAs in vivo and stimulates their degradation by the RRP6 exosome subunit in vitro. Moreover, RRP6 depletion also leads to accumulation of small RNAs in vivo. We propose that MUT68 and RRP6 cooperate in the degradation of mature miRNAs and siRNAs, as a quality control mechanism to eliminate dysfunctional or damaged small RNA molecules.

KW - Exosome

KW - RNA interference

KW - miRNA quality control

UR - http://www.scopus.com/inward/record.url?scp=77649255166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649255166&partnerID=8YFLogxK

U2 - 10.1073/pnas.0912632107

DO - 10.1073/pnas.0912632107

M3 - Article

VL - 107

SP - 3906

EP - 3911

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 8

ER -