Urethral Carcinoma and Hyperplasia in Male and Female B6C3F1 Mice Treated With 3,3′,4,4′-Tetrachloroazobenzene (TCAB)

B. P. Singh, A. Nyska, G. E. Kissling, W. Lieuallen, S. L. Johansson, Sonny Lennart Johansson, M. J. Hooth

Research output: Contribution to journalArticle

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Abstract

B6C3F1 mice chronically exposed to 3,3′,4,4′-tetrachloroazobenzene (TCAB), a contaminant of dichloroaniline-derived herbicides, developed a number of neoplastic and nonneoplastic lesions, including carcinoma of the urinary tract. Groups of fifty male and fifty female B6C3F1 mice were exposed by gavage to TCAB at dose levels of 0, 3, 10, and 30 mg/kg five days a week for two years. Control animals received corn oil:acetone (99:1) vehicle. Decreased survival of male mice in the mid-dose group and of male and female mice in the high-dose groups was related mainly to the occurrence of urethral transitional cell (urothelial) carcinoma and resulting urinary obstruction. Increased urethral transitional cell carcinomas were seen in all treated male groups in a dose-related manner as well as in the females treated with 30 mg/kg TCAB. Administration of TCAB was also associated with increased transitional cell hyperplasia of the urethra. Most nonneoplastic lesions of the urogenital tract were considered secondary to local invasion and urinary obstruction by the urethral transitional cell carcinomas. The mechanism of tumor induction is uncertain, but the high frequency of tumors in the proximal urethra of male mice suggests that the neoplasms result from the exposure of a susceptible population of urothelial cells to a carcinogenic metabolite of TCAB.

Original languageEnglish (US)
Pages (from-to)372-381
Number of pages10
JournalToxicologic Pathology
Volume38
Issue number3
DOIs
StatePublished - Jan 1 2010

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Hyperplasia
Cells
Transitional Cell Carcinoma
Carcinoma
Tumors
Urethra
Corn Oil
Herbicides
Metabolites
Acetone
Urethral Obstruction
Animals
Neoplasms
Impurities
Urinary Tract
Population

Keywords

  • 3, 3′, 4, 4′-tetrachloroazobenzene (TCAB)
  • carcinoma
  • mice
  • transitional cell carcinoma
  • urethra

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Singh, B. P., Nyska, A., Kissling, G. E., Lieuallen, W., Johansson, S. L., Johansson, S. L., & Hooth, M. J. (2010). Urethral Carcinoma and Hyperplasia in Male and Female B6C3F1 Mice Treated With 3,3′,4,4′-Tetrachloroazobenzene (TCAB). Toxicologic Pathology, 38(3), 372-381. https://doi.org/10.1177/0192623310362708

Urethral Carcinoma and Hyperplasia in Male and Female B6C3F1 Mice Treated With 3,3′,4,4′-Tetrachloroazobenzene (TCAB). / Singh, B. P.; Nyska, A.; Kissling, G. E.; Lieuallen, W.; Johansson, S. L.; Johansson, Sonny Lennart; Hooth, M. J.

In: Toxicologic Pathology, Vol. 38, No. 3, 01.01.2010, p. 372-381.

Research output: Contribution to journalArticle

Singh, BP, Nyska, A, Kissling, GE, Lieuallen, W, Johansson, SL, Johansson, SL & Hooth, MJ 2010, 'Urethral Carcinoma and Hyperplasia in Male and Female B6C3F1 Mice Treated With 3,3′,4,4′-Tetrachloroazobenzene (TCAB)', Toxicologic Pathology, vol. 38, no. 3, pp. 372-381. https://doi.org/10.1177/0192623310362708
Singh, B. P. ; Nyska, A. ; Kissling, G. E. ; Lieuallen, W. ; Johansson, S. L. ; Johansson, Sonny Lennart ; Hooth, M. J. / Urethral Carcinoma and Hyperplasia in Male and Female B6C3F1 Mice Treated With 3,3′,4,4′-Tetrachloroazobenzene (TCAB). In: Toxicologic Pathology. 2010 ; Vol. 38, No. 3. pp. 372-381.
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