Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection

Chunyi Zhou, Fatema Bhinderwala, McKenzie K. Lehman, Vinai C. Thomas, Sujata S. Chaudhari, Kelsey J. Yamada, Kirk W. Foster, Robert Powers, Tammy Kielian, Paul D. Fey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH 3 and CO 2 from urea, is key to pH homeostasis in bacterial pathogens under acidic stress and nitrogen limitation. However, the function of urease in S. aureus niche colonization and nitrogen metabolism has not been extensively studied. We discovered that urease is essential for pH homeostasis and viability in urea-rich environments under weak acid stress. The regulation of urease transcription by CcpA, Agr, and CodY was identified in this study, implying a complex network that controls urease expression in response to changes in metabolic flux. In addition, it was determined that the endogenous urea derived from arginine is not a significant contributor to the intracellular nitrogen pool in non-acidic conditions. Furthermore, we found that during a murine chronic renal infection, urease facilitates S. aureus persistence by promoting bacterial fitness in the low-pH, urea-rich kidney. Overall, our study establishes that urease in S. aureus is not only a primary component of the acid response network but also an important factor required for persistent murine renal infections.

Original languageEnglish (US)
Article numbere1007538
JournalPLoS pathogens
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2019

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Urease
Staphylococcus aureus
Kidney
Acids
Infection
Urea
Nitrogen
Homeostasis
Carbon Monoxide
Arginine
Morbidity
Enzymes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection. / Zhou, Chunyi; Bhinderwala, Fatema; Lehman, McKenzie K.; Thomas, Vinai C.; Chaudhari, Sujata S.; Yamada, Kelsey J.; Foster, Kirk W.; Powers, Robert; Kielian, Tammy; Fey, Paul D.

In: PLoS pathogens, Vol. 15, No. 1, e1007538, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Chaudhari, Sujata S.

AU - Yamada, Kelsey J.

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