Urea-cysteine based extraction of densely crosslinked proteins from sorghum distillers grains with high yield and quality

Wei Li, Bingnan Mu, Helan Xu, Lan Xu, Yiqi Yang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A urea-cysteine based system is developed to extract densely crosslinked sorghum proteins from sorghum distillers grains with high efficiency and quality. Due to the high crosslinking degrees, sorghum proteins are expected to possess better tensile properties and aqueous stabilities than common proteins, thus become a promising candidate to replace the unsustainable petroleum products. However, due to the long-standing difficulty in dissolution of densely crosslinked proteins, to extract sorghum proteins with high efficiency and quality for satisfactory properties in end products has not been achieved by previous methods In this paper, a urea-cysteine based system is used to replace the most widely used ethanol based system. The urea-cysteine based system endows sorghum proteins with high purity and yield, which are 14% and 42% higher than those from the ethanol based system. Based on amine and thiol group analysis, the urea-cysteine based system provides sorghum proteins with preserved peptide backbones, full breakage of disulfide bonds for linear structures and sufficient thiol groups for re-oxidation. Due to such unique structures and thus regular arrangement of protein molecules during film casting, sorghum protein films from the urea-cysteine based system have crystallinity 21% higher than those from the ethanol based system. Tensile properties and water-vapor-barrier properties of sorghum protein films are improved by 27% and 28% compared to the ethanol based system. Sorghum protein films derived from sorghum distillers grains are first reported and their tensile properties are better than those of all sorghum protein films developed previously. Quantified effects of preservation of protein peptide backbones and recovery of disulfide bonds on tensile strength are also compared. With the unique structures, sorghum proteins from the urea-cysteine based system have great potential to achieve excellent properties in various applications, such as films and fibers, which account for a total market share of over $100 billion worldwide.

Original languageEnglish (US)
Pages (from-to)360-371
Number of pages12
JournalIndustrial Crops and Products
Volume121
DOIs
StatePublished - Oct 1 2018

Fingerprint

sorghum protein
distillers grains
cysteine
urea
proteins
ethanol
disulfide bonds
thiols
peptides
market share
extracts
tensile strength
amines
crosslinking
water vapor
petroleum
purity

Keywords

  • Highly crosslinked proteins
  • Protein extraction
  • Protein films
  • Sorghum distillers grains
  • Sorghum protein
  • Urea solution

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Urea-cysteine based extraction of densely crosslinked proteins from sorghum distillers grains with high yield and quality. / Li, Wei; Mu, Bingnan; Xu, Helan; Xu, Lan; Yang, Yiqi.

In: Industrial Crops and Products, Vol. 121, 01.10.2018, p. 360-371.

Research output: Contribution to journalArticle

@article{e64d82b01e744190a6a140e7bc440ff3,
title = "Urea-cysteine based extraction of densely crosslinked proteins from sorghum distillers grains with high yield and quality",
abstract = "A urea-cysteine based system is developed to extract densely crosslinked sorghum proteins from sorghum distillers grains with high efficiency and quality. Due to the high crosslinking degrees, sorghum proteins are expected to possess better tensile properties and aqueous stabilities than common proteins, thus become a promising candidate to replace the unsustainable petroleum products. However, due to the long-standing difficulty in dissolution of densely crosslinked proteins, to extract sorghum proteins with high efficiency and quality for satisfactory properties in end products has not been achieved by previous methods In this paper, a urea-cysteine based system is used to replace the most widely used ethanol based system. The urea-cysteine based system endows sorghum proteins with high purity and yield, which are 14{\%} and 42{\%} higher than those from the ethanol based system. Based on amine and thiol group analysis, the urea-cysteine based system provides sorghum proteins with preserved peptide backbones, full breakage of disulfide bonds for linear structures and sufficient thiol groups for re-oxidation. Due to such unique structures and thus regular arrangement of protein molecules during film casting, sorghum protein films from the urea-cysteine based system have crystallinity 21{\%} higher than those from the ethanol based system. Tensile properties and water-vapor-barrier properties of sorghum protein films are improved by 27{\%} and 28{\%} compared to the ethanol based system. Sorghum protein films derived from sorghum distillers grains are first reported and their tensile properties are better than those of all sorghum protein films developed previously. Quantified effects of preservation of protein peptide backbones and recovery of disulfide bonds on tensile strength are also compared. With the unique structures, sorghum proteins from the urea-cysteine based system have great potential to achieve excellent properties in various applications, such as films and fibers, which account for a total market share of over $100 billion worldwide.",
keywords = "Highly crosslinked proteins, Protein extraction, Protein films, Sorghum distillers grains, Sorghum protein, Urea solution",
author = "Wei Li and Bingnan Mu and Helan Xu and Lan Xu and Yiqi Yang",
year = "2018",
month = "10",
day = "1",
doi = "10.1016/j.indcrop.2018.05.035",
language = "English (US)",
volume = "121",
pages = "360--371",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",

}

TY - JOUR

T1 - Urea-cysteine based extraction of densely crosslinked proteins from sorghum distillers grains with high yield and quality

AU - Li, Wei

AU - Mu, Bingnan

AU - Xu, Helan

AU - Xu, Lan

AU - Yang, Yiqi

PY - 2018/10/1

Y1 - 2018/10/1

N2 - A urea-cysteine based system is developed to extract densely crosslinked sorghum proteins from sorghum distillers grains with high efficiency and quality. Due to the high crosslinking degrees, sorghum proteins are expected to possess better tensile properties and aqueous stabilities than common proteins, thus become a promising candidate to replace the unsustainable petroleum products. However, due to the long-standing difficulty in dissolution of densely crosslinked proteins, to extract sorghum proteins with high efficiency and quality for satisfactory properties in end products has not been achieved by previous methods In this paper, a urea-cysteine based system is used to replace the most widely used ethanol based system. The urea-cysteine based system endows sorghum proteins with high purity and yield, which are 14% and 42% higher than those from the ethanol based system. Based on amine and thiol group analysis, the urea-cysteine based system provides sorghum proteins with preserved peptide backbones, full breakage of disulfide bonds for linear structures and sufficient thiol groups for re-oxidation. Due to such unique structures and thus regular arrangement of protein molecules during film casting, sorghum protein films from the urea-cysteine based system have crystallinity 21% higher than those from the ethanol based system. Tensile properties and water-vapor-barrier properties of sorghum protein films are improved by 27% and 28% compared to the ethanol based system. Sorghum protein films derived from sorghum distillers grains are first reported and their tensile properties are better than those of all sorghum protein films developed previously. Quantified effects of preservation of protein peptide backbones and recovery of disulfide bonds on tensile strength are also compared. With the unique structures, sorghum proteins from the urea-cysteine based system have great potential to achieve excellent properties in various applications, such as films and fibers, which account for a total market share of over $100 billion worldwide.

AB - A urea-cysteine based system is developed to extract densely crosslinked sorghum proteins from sorghum distillers grains with high efficiency and quality. Due to the high crosslinking degrees, sorghum proteins are expected to possess better tensile properties and aqueous stabilities than common proteins, thus become a promising candidate to replace the unsustainable petroleum products. However, due to the long-standing difficulty in dissolution of densely crosslinked proteins, to extract sorghum proteins with high efficiency and quality for satisfactory properties in end products has not been achieved by previous methods In this paper, a urea-cysteine based system is used to replace the most widely used ethanol based system. The urea-cysteine based system endows sorghum proteins with high purity and yield, which are 14% and 42% higher than those from the ethanol based system. Based on amine and thiol group analysis, the urea-cysteine based system provides sorghum proteins with preserved peptide backbones, full breakage of disulfide bonds for linear structures and sufficient thiol groups for re-oxidation. Due to such unique structures and thus regular arrangement of protein molecules during film casting, sorghum protein films from the urea-cysteine based system have crystallinity 21% higher than those from the ethanol based system. Tensile properties and water-vapor-barrier properties of sorghum protein films are improved by 27% and 28% compared to the ethanol based system. Sorghum protein films derived from sorghum distillers grains are first reported and their tensile properties are better than those of all sorghum protein films developed previously. Quantified effects of preservation of protein peptide backbones and recovery of disulfide bonds on tensile strength are also compared. With the unique structures, sorghum proteins from the urea-cysteine based system have great potential to achieve excellent properties in various applications, such as films and fibers, which account for a total market share of over $100 billion worldwide.

KW - Highly crosslinked proteins

KW - Protein extraction

KW - Protein films

KW - Sorghum distillers grains

KW - Sorghum protein

KW - Urea solution

UR - http://www.scopus.com/inward/record.url?scp=85047308192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047308192&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2018.05.035

DO - 10.1016/j.indcrop.2018.05.035

M3 - Article

AN - SCOPUS:85047308192

VL - 121

SP - 360

EP - 371

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -