Uptake and utilization of lyso-phosphatidylethanolamine by Saccharomyces cerevisiae

Wayne R. Riekhof, Dennis R. Voelker

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Phosphatidylethanolamine (PtdEtn) is synthesized by multiple pathways located in different subcellular compartments in yeast. Strains defective in the synthesis of PtdEtn via phosphatidylserine (PtdSer) synthase/decarboxylase are auxotrophic for ethanolamine, which must be transported into the cell and converted to phospholipid by the cytidinediphosphate-ethanolamine-dependent Kennedy pathway. We now demonstrate that yeast strains with psd1Δ psd2Δ mutations, devoid of PtdSer decarboxylases, import and acylate exogenous 1-acyl-2-hydroxyl-sn-glycero-3-phosphoethanolamine (lyso-PtdEtn). Lyso-PtdEtn supports growth and replaces the mitochondrial pool of PtdEtn much more efficiently than and independently of PtdEtn derived from the Kennedy pathway. Deletion of both the PtdSer decarboxylase and Kennedy pathways yields a strain that is a stringent lyso-PtdEtn auxotroph. Evidence for the specific uptake of lyso-PtdEtn by yeast comes from analysis of strains harboring deletions of the aminophospholipid translocating P-type ATPases (APLTs). Elimination of the APLTs, Dnf1p and Dnf2p, or their noncatalytic β-subunit, Lem3p, blocked the import of radiolabeled lyso-PtdEtn and resulted in growth inhibition of lyso-PtdEtn auxotrophs. In cell extracts, lyso-PtdEtn is rapidly converted to PtdEtn by an acyl-CoA-dependent acyl-transferase. These results now provide 1) an assay for APLT function based on an auxotrophic phenotype, 2) direct demonstration of APLT action on a physiologically relevant substrate, and 3) a genetic screen aimed at finding additional components that mediate the internalization, trafficking, and acylation of exogenous lyso-phospholipids.

Original languageEnglish (US)
Pages (from-to)36588-36596
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number48
DOIs
StatePublished - Dec 1 2006

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Yeast
Saccharomyces cerevisiae
Ethanolamine
Yeasts
phosphatidylethanolamine
CDPdiacylglycerol-Serine O-Phosphatidyltransferase
Phospholipids
Acylation
Acyl Coenzyme A
Carboxy-Lyases
Growth
Transferases
Cell Extracts
Hydroxyl Radical
Adenosine Triphosphatases
Assays
Demonstrations
Phenotype
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Uptake and utilization of lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. / Riekhof, Wayne R.; Voelker, Dennis R.

In: Journal of Biological Chemistry, Vol. 281, No. 48, 01.12.2006, p. 36588-36596.

Research output: Contribution to journalArticle

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