Uptake and excretion of Brucella abortus in tissues of the face fly (Musca autumnalis).

N. F. Cheville, Douglas G Rogers, W. L. Deyoe, E. S. Krafsur, J. C. Cheville

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To determine their capacity to host Brucella abortus, face flies were examined 1 to 120 hours after feeding on broth containing bacteria and bovine erythrocytes. Brucella abortus was cultured in large numbers from whole flies for 12 hours after feeding, but not after 72 hours. Histologic analysis showed that brucellae were rapidly taken into the midgut, sequestered from erythrocytes, transiently stored, and shed in the feces; there was no evidence of bacterial replication within epithelial cells. Immunoperoxidase and immunogold techniques revealed that most brucellae in the gut were confined to the lumen by the peritrophic membrane, that brucellae were degraded in secondary lysosomes of midgut epithelial cells, and that intact brucellae passed into connective tissues surrounding the midgut. Bacterial excretion without midgut replication is consistent with transient, but not long-term, insect transmission in nature.

Original languageEnglish (US)
Pages (from-to)1302-1306
Number of pages5
JournalAmerican journal of veterinary research
Volume50
Issue number8
StatePublished - Jan 1 1989

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Muscidae
Musca autumnalis
Brucella abortus
Brucella
Brucella melitensis biovar Abortus
midgut
excretion
uptake mechanisms
epithelial cells
erythrocytes
Erythrocytes
Epithelial Cells
peritrophic membrane
sheds
lysosomes
Lysosomes
Immunoenzyme Techniques
Feces
Diptera
connective tissues

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cheville, N. F., Rogers, D. G., Deyoe, W. L., Krafsur, E. S., & Cheville, J. C. (1989). Uptake and excretion of Brucella abortus in tissues of the face fly (Musca autumnalis). American journal of veterinary research, 50(8), 1302-1306.

Uptake and excretion of Brucella abortus in tissues of the face fly (Musca autumnalis). / Cheville, N. F.; Rogers, Douglas G; Deyoe, W. L.; Krafsur, E. S.; Cheville, J. C.

In: American journal of veterinary research, Vol. 50, No. 8, 01.01.1989, p. 1302-1306.

Research output: Contribution to journalArticle

Cheville, NF, Rogers, DG, Deyoe, WL, Krafsur, ES & Cheville, JC 1989, 'Uptake and excretion of Brucella abortus in tissues of the face fly (Musca autumnalis).', American journal of veterinary research, vol. 50, no. 8, pp. 1302-1306.
Cheville NF, Rogers DG, Deyoe WL, Krafsur ES, Cheville JC. Uptake and excretion of Brucella abortus in tissues of the face fly (Musca autumnalis). American journal of veterinary research. 1989 Jan 1;50(8):1302-1306.
Cheville, N. F. ; Rogers, Douglas G ; Deyoe, W. L. ; Krafsur, E. S. ; Cheville, J. C. / Uptake and excretion of Brucella abortus in tissues of the face fly (Musca autumnalis). In: American journal of veterinary research. 1989 ; Vol. 50, No. 8. pp. 1302-1306.
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