Abstract
The lin-12/Notch signaling pathway is conserved from worms to humans and is a master regulator of metazoan development. Here, we demonstrate that lin-12/Notch gain-of-function (gf) animals display precocious alae at the L4 larval stage with a significant increase in let-7 expression levels. Furthermore, lin-12(gf) animals display a precocious and higher level of let-7 gfp transgene expression in seam cells at L3 stage. Interestingly, lin-12(gf) mutant rescued the lethal phenotype of let-7 mutants similar to other known heterochronic mutants. We propose that lin-12/Notch signaling pathway functions in late developmental timing, upstream of or in parallel to the let-7 heterochronic pathway. Importantly, the human microRNA let-7a was also upregulated in various human cell lines in response to Notch1 activation, suggesting an evolutionarily conserved cross-talk between let-7 and the canonical lin-12/Notch signaling pathway.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 191-199 |
| Number of pages | 9 |
| Journal | Developmental Biology |
| Volume | 316 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 15 2008 |
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Keywords
- Cell fate
- Developmental timing
- Heterochronic genes
- Seam cells
- let-7
- lin-12/Notch
- lin-41
- microRNA molting
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. / Solomon, Aharon; Mian, Yousaf; Ortega-Cava, Cesar; Liu, Victor Won Tat; Gurumurthy, Channabasavaiah B; Naramura, Mayumi; Band, Vimla; Band, Hamid.
In: Developmental Biology, Vol. 316, No. 2, 15.04.2008, p. 191-199.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants
AU - Solomon, Aharon
AU - Mian, Yousaf
AU - Ortega-Cava, Cesar
AU - Liu, Victor Won Tat
AU - Gurumurthy, Channabasavaiah B
AU - Naramura, Mayumi
AU - Band, Vimla
AU - Band, Hamid
PY - 2008/4/15
Y1 - 2008/4/15
N2 - The lin-12/Notch signaling pathway is conserved from worms to humans and is a master regulator of metazoan development. Here, we demonstrate that lin-12/Notch gain-of-function (gf) animals display precocious alae at the L4 larval stage with a significant increase in let-7 expression levels. Furthermore, lin-12(gf) animals display a precocious and higher level of let-7 gfp transgene expression in seam cells at L3 stage. Interestingly, lin-12(gf) mutant rescued the lethal phenotype of let-7 mutants similar to other known heterochronic mutants. We propose that lin-12/Notch signaling pathway functions in late developmental timing, upstream of or in parallel to the let-7 heterochronic pathway. Importantly, the human microRNA let-7a was also upregulated in various human cell lines in response to Notch1 activation, suggesting an evolutionarily conserved cross-talk between let-7 and the canonical lin-12/Notch signaling pathway.
AB - The lin-12/Notch signaling pathway is conserved from worms to humans and is a master regulator of metazoan development. Here, we demonstrate that lin-12/Notch gain-of-function (gf) animals display precocious alae at the L4 larval stage with a significant increase in let-7 expression levels. Furthermore, lin-12(gf) animals display a precocious and higher level of let-7 gfp transgene expression in seam cells at L3 stage. Interestingly, lin-12(gf) mutant rescued the lethal phenotype of let-7 mutants similar to other known heterochronic mutants. We propose that lin-12/Notch signaling pathway functions in late developmental timing, upstream of or in parallel to the let-7 heterochronic pathway. Importantly, the human microRNA let-7a was also upregulated in various human cell lines in response to Notch1 activation, suggesting an evolutionarily conserved cross-talk between let-7 and the canonical lin-12/Notch signaling pathway.
KW - Cell fate
KW - Developmental timing
KW - Heterochronic genes
KW - Seam cells
KW - let-7
KW - lin-12/Notch
KW - lin-41
KW - microRNA molting
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UR - http://www.scopus.com/inward/citedby.url?scp=41149154762&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2007.12.046
DO - 10.1016/j.ydbio.2007.12.046
M3 - Article
VL - 316
SP - 191
EP - 199
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -