Unnatural amino acid substitution as a probe of the allosteric coupling pathway in a mycobacterial Cu(I) sensor

Zhen Ma, Darin M. Cowart, Brian P. Ward, Randy J. Arnold, Richard D. DiMarchi, Limei Zhang, Graham N. George, Robert A. Scott, David P. Giedroc

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

(Chemical Equation Presented) The Cu(I) sensor Mycobacterium tuberculosis CsoR is a founding member of a new metalloregulatory protein family. Here we show that two "atom" substitutions of the NB2 face of a Cu(I) coordinating histisine-61 allosterically uncouple Cu(I) and DNA binding, with no effect on Cu(I) binding affinity and coordination structure. A model analogous to the allosteric switch mechanism in Staphylococcus aureus CzrA, a zinc sensor protein with a completely different fold, is proposed.

Original languageEnglish (US)
Pages (from-to)18044-18045
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number50
DOIs
StatePublished - Dec 23 2009

Fingerprint

Amino Acid Substitution
Amino acids
Substitution reactions
Proteins
Amino Acids
Sensors
Mycobacterium tuberculosis
Staphylococcus aureus
Zinc
DNA
Switches
Atoms

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Unnatural amino acid substitution as a probe of the allosteric coupling pathway in a mycobacterial Cu(I) sensor. / Ma, Zhen; Cowart, Darin M.; Ward, Brian P.; Arnold, Randy J.; DiMarchi, Richard D.; Zhang, Limei; George, Graham N.; Scott, Robert A.; Giedroc, David P.

In: Journal of the American Chemical Society, Vol. 131, No. 50, 23.12.2009, p. 18044-18045.

Research output: Contribution to journalArticle

Ma, Z, Cowart, DM, Ward, BP, Arnold, RJ, DiMarchi, RD, Zhang, L, George, GN, Scott, RA & Giedroc, DP 2009, 'Unnatural amino acid substitution as a probe of the allosteric coupling pathway in a mycobacterial Cu(I) sensor', Journal of the American Chemical Society, vol. 131, no. 50, pp. 18044-18045. https://doi.org/10.1021/ja908372b
Ma, Zhen ; Cowart, Darin M. ; Ward, Brian P. ; Arnold, Randy J. ; DiMarchi, Richard D. ; Zhang, Limei ; George, Graham N. ; Scott, Robert A. ; Giedroc, David P. / Unnatural amino acid substitution as a probe of the allosteric coupling pathway in a mycobacterial Cu(I) sensor. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 50. pp. 18044-18045.
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