Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis

Josh T. Cuperus, Alberto Carbonell, Noah Fahlgren, Hernan Garcia Ruiz, Russell T. Burke, Atsushi Takeda, Christopher M. Sullivan, Sunny D. Gilbert, Taiowa A. Montgomery, James C. Carrington

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNAs (miRNAs). Here, Arabidopsis thaliana secondary siRNAs from mRNA as well as trans-acting siRNAs are shown to be triggered through initial targeting by a 22-nucleotide (nt) miRNA that associates with AGO1. In contrast to canonical 21-nt miRNAs, 22-nt miRNAs primarily arise from foldback precursors containing asymmetric bulges. Using artificial miRNA constructs, conversion of asymmetric foldbacks to symmetric foldbacks resulted in the production of 21-nt forms of miR173, miR472 and miR828. Both 21-and 22-nt forms associated with AGO1 and guided accurate slicer activity, but only 22-nt forms were competent to trigger RDR6-dependent siRNA production from target RNA. These data suggest that AGO1 functions differentially with 21-and 22-nt miRNAs to engage the RDR6-associated amplification apparatus.

Original languageEnglish (US)
Pages (from-to)997-1003
Number of pages7
JournalNature Structural and Molecular Biology
Volume17
Issue number8
DOIs
StatePublished - Aug 1 2010

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MicroRNAs
Arabidopsis
Small Interfering RNA
Nucleotides
RNA Replicase
RNA Interference
RNA
Messenger RNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. / Cuperus, Josh T.; Carbonell, Alberto; Fahlgren, Noah; Garcia Ruiz, Hernan; Burke, Russell T.; Takeda, Atsushi; Sullivan, Christopher M.; Gilbert, Sunny D.; Montgomery, Taiowa A.; Carrington, James C.

In: Nature Structural and Molecular Biology, Vol. 17, No. 8, 01.08.2010, p. 997-1003.

Research output: Contribution to journalArticle

Cuperus, JT, Carbonell, A, Fahlgren, N, Garcia Ruiz, H, Burke, RT, Takeda, A, Sullivan, CM, Gilbert, SD, Montgomery, TA & Carrington, JC 2010, 'Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis', Nature Structural and Molecular Biology, vol. 17, no. 8, pp. 997-1003. https://doi.org/10.1038/nsmb.1866
Cuperus, Josh T. ; Carbonell, Alberto ; Fahlgren, Noah ; Garcia Ruiz, Hernan ; Burke, Russell T. ; Takeda, Atsushi ; Sullivan, Christopher M. ; Gilbert, Sunny D. ; Montgomery, Taiowa A. ; Carrington, James C. / Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. In: Nature Structural and Molecular Biology. 2010 ; Vol. 17, No. 8. pp. 997-1003.
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