Unconventional secretion of Ebola virus matrix protein VP40

Olivier Reynard, St Patrick Reid, Audrey Page, Mathieu Mateo, Nathalie Alazard-Dany, Hervé Raoul, Christopher F. Basler, Viktor E. Volchkov

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Ebola virus matrix protein VP40 plays an essential role in virus assembly and budding. In this study we reveal that transient VP40 expression results in the release into the culture medium of substantial amounts of soluble monomeric VP40 in addition to the release of virus-like particles containing an oligomeric form of this protein as previously described. We show that VP40 secretion is endoplasmic reticulum/Golgi-independent and is not associated with cell death. Soluble VP40 was observed during Ebola virus infection of cells and was also found in the serum of virus-infected animals albeit in lower amounts. Unconventional secretion of VP40 may therefore play a role in Ebola virus pathogenicity.

Original languageEnglish (US)
JournalJournal of Infectious Diseases
Volume204
Issue numberSUPPL. 3
DOIs
StatePublished - Nov 1 2011

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Ebolavirus
Ebola Hemorrhagic Fever
Virus Release
Virus Assembly
Endoplasmic Reticulum
Virion
Virulence
Culture Media
Proteins
Cell Death
Viruses
Serum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Reynard, O., Reid, S. P., Page, A., Mateo, M., Alazard-Dany, N., Raoul, H., ... Volchkov, V. E. (2011). Unconventional secretion of Ebola virus matrix protein VP40. Journal of Infectious Diseases, 204(SUPPL. 3). https://doi.org/10.1093/infdis/jir305

Unconventional secretion of Ebola virus matrix protein VP40. / Reynard, Olivier; Reid, St Patrick; Page, Audrey; Mateo, Mathieu; Alazard-Dany, Nathalie; Raoul, Hervé; Basler, Christopher F.; Volchkov, Viktor E.

In: Journal of Infectious Diseases, Vol. 204, No. SUPPL. 3, 01.11.2011.

Research output: Contribution to journalArticle

Reynard, O, Reid, SP, Page, A, Mateo, M, Alazard-Dany, N, Raoul, H, Basler, CF & Volchkov, VE 2011, 'Unconventional secretion of Ebola virus matrix protein VP40', Journal of Infectious Diseases, vol. 204, no. SUPPL. 3. https://doi.org/10.1093/infdis/jir305
Reynard, Olivier ; Reid, St Patrick ; Page, Audrey ; Mateo, Mathieu ; Alazard-Dany, Nathalie ; Raoul, Hervé ; Basler, Christopher F. ; Volchkov, Viktor E. / Unconventional secretion of Ebola virus matrix protein VP40. In: Journal of Infectious Diseases. 2011 ; Vol. 204, No. SUPPL. 3.
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